IL-4 antibody

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P ResetRabbit anti Human Interleukin-4
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-4
Rabbit anti Human Interleukin-4 antibody recognizes human IL-4 (Interleukin-4), also known as B-cell stimulatory factor 1, a 129 amino acid pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching. IL-4 signalling through the IL-4 receptor (IL-4R), under the influence of Stat6, induces the differentiation of CD4+ T cells into Th2 helper cells, which themselves then secrete IL-4 along with IL-5, IL-6, IL-10 and IL-13. Excessive secretion of IL-4 by Th2 helper cells has been linked with allergy and elevated IgE levels. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide. - Antiserum Preparation
- Antisera to human IL-4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-4
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
0.5 | 2.0ug/ml |
Functional Assays | ![]() |
0.03 | 0.05ug/ml |
Immunohistology - Paraffin 1 | ![]() |
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Western Blotting | ![]() |
0.1 | 0.2ug/ml |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
- ELISA
- This purified human IL-4 antibody may be used in ELISA with recombinant human IL-4 (PHP234) as the standard.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Description | Sheep anti Rabbit IgG:RPE | ||||||
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Description | Sheep anti Rabbit IgG:FITC | ||||||
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):HRP |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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Description | Antigen Retrieval Buffer, pH8.0 | ||||||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
List Price | Your Price | ||||||
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Description | TidyBlot Western Blot Detection Reagent:HRP |
Further Reading
-
Arai, N. et al. (1989) Complete nucleotide sequence of the chromosomal gene for human IL-4 and its expression.
J Immunol. 142 (1): 274-82. -
Steinke, J.W. & Borish, L. (2001) Th2 cytokines and asthma. Interleukin-4: its role in the pathogenesis of asthma, and targeting it for asthma treatment with interleukin-4 receptor antagonists.
Respir Res. 2 (2): 66-70.
- RRID
- AB_609743
- UniProt
- P05112
- Entrez Gene
- IL4
- GO Terms
- GO:0005125 cytokine activity
- GO:0005136 interleukin-4 receptor binding
- GO:0005615 extracellular space
- GO:0006935 chemotaxis
- GO:0006968 cellular defense response
- GO:0008083 growth factor activity
- GO:0008203 cholesterol metabolic process
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- View More GO Terms
- GO:0030183 B cell differentiation
- GO:0030890 positive regulation of B cell proliferation
- GO:0042092 type 2 immune response
- GO:0043066 negative regulation of apoptosis
- GO:0045189 connective tissue growth factor biosynthetic process
- GO:0045348 positive regulation of MHC class II biosynthetic process
- GO:0045671 negative regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048295 positive regulation of isotype switching to IgE isotypes
- GO:0048304 positive regulation of isotype switching to IgG isotypes
AHP780

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