Rabbit anti Human Interleukin-4 antibody recognizes human IL-4 (Interleukin-4), also known as B-cell stimulatory factor 1, a 129 amino acid pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching.
IL-4 signalling through the IL-4 receptor (IL-4R), under the influence of Stat6, induces the differentiation of CD4+ T cells into Th2 helper cells, which themselves then secrete IL-4 along with IL-5, IL-6, IL-10 and IL-13. Excessive secretion of IL-4 by Th2 helper cells has been linked with allergy and elevated IgE levels.
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human IL-4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant human IL-4
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution.
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of reconstitution.
- Entrez Gene
- GO Terms
interleukin-4 receptor binding
cellular defense response
growth factor activity
cholesterol metabolic process
transcription activator activity
transcription repressor activity
B cell differentiation
positive regulation of B cell proliferation
type 2 immune response
negative regulation of apoptosis
connective tissue growth factor biosynthetic process
positive regulation of MHC class II biosynthetic process
negative regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-4 antibody
|Immunohistology - Paraffin 1
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- This purified human IL-4 antibody may be used in ELISA with recombinant human IL-4 (PHP234) as the standard.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Arai, N. et al. (1989) Complete nucleotide sequence of the chromosomal gene for human IL-4 and its expression.
J Immunol. 142 (1): 274-82.
Steinke, J.W. & Borish, L. (2001) Th2 cytokines and asthma. Interleukin-4: its role in the pathogenesis of asthma, and targeting it for asthma treatment with interleukin-4 receptor antagonists.
Respir Res. 2 (2): 66-70.