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IL-27 Receptor Alpha antibody

Rabbit anti Human IL-27 Receptor Alpha (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-27 Receptor Alpha
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1620T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF P * WB 50 µg loader
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loader
AHP1620
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF P * WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti human IL-27Rα antibody recognizes the carboxy terminal of the alpha subunit of the human IL-27 receptor, otherwise known as the T cell cytokine receptor (TCCR). It is a member of the type I cytokine receptor family with higher levels of expression in CD4+ T cells than in CD8+ T cells. It is a receptor for Interleukin-27 and is involved in the regulation of Th1-type T cells. It also appears to be involved in innate defense mechanisms. It is highly expressed in lymphoid tissue such as spleen, lymph nodes and peripheral blood leukocytes. It is also weakly expressed in tissues such as the heart, brain and lung.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
AHP1620T: Antiserum to human Interleukin receptor alpha (C-Terminal) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
AHP1620: Antiserum to human IL-27Rα (C-Terminal) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
AHP1620T: <0.1% Sodium Azide (NaN3)
AHP1620: 0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids near the carboxy terminus of human IL-27Rα precursor.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 20ug/ml
Immunohistology - Paraffin 1 5.0ug/ml Pack Size: 50 µg
5ug/ml Pack Size: 0.1 mg
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti human IL-27Rα detects a band of approximately 70kDa in human spleen tissue lysates.

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Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
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Description Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB * 0.5 ml
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Description TidyBlot Western Blot Detection Reagent:HRP

Synonyms
TCCR
RRID
AB_2295940
UniProt
Q6UWB1
Entrez Gene
IL27RA
GO Terms
GO:0005887 integral to plasma membrane
GO:0006955 immune response

AHP1620T

154640

AHP1620

160802 160927

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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