IL-27 Receptor Alpha antibody

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Rabbit anti Human IL-27 Receptor Alpha (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1620T
Datasheet
IF P* WB
MSDS
50 µg loader

AHP1620
Datasheet
IF P* WB
MSDS
0.1 mg loader

Rabbit anti human IL-27Rα antibody recognizes the carboxy terminal of the alpha subunit of the human IL-27 receptor, otherwise known as the T cell cytokine receptor (TCCR). It is a member of the type I cytokine receptor family with higher levels of expression in CD4+ T cells than in CD8+ T cells. It is a receptor for Interleukin-27 and is involved in the regulation of Th1-type T cells. It also appears to be involved in innate defense mechanisms. It is highly expressed in lymphoid tissue such as spleen, lymph nodes and peripheral blood leukocytes. It is also weakly expressed in tissues such as the heart, brain and lung.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human IL-27Rα (C-Terminal) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 50 µg
0.09% Sodium Azide (NaN3)
Pack Size: 0.1 mg
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids near the carboxy terminus of human IL-27Rα precursor.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q6UWB1
Entrez Gene
IL27RA
GO Terms
GO:0005887 integral to plasma membrane
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IL-27 Receptor Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 20ug/ml
Immunohistology - Paraffin 1 5.0ug/ml Pack Size: 50 µg
5ug/ml Pack Size: 0.1 mg
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti human IL-27Rα detects a band of approximately 70kDa in human spleen tissue lysates.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml