IL-21 antibody

100% Secure


Rabbit anti Interleukin-21

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00596
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human interleukin-21 antibody recognizes interleukin-21 (IL-21), also known as Za11. IL-21 is a 133 amnio acid ~ 17 kDa immunoregulatory cytokine.

The IL21 gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of IL21 plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Mutation of the IL21 gene causes Immunodeficiency, common variable, 11 (CVID11), a condition characterized by antibody and the inability to mount an antibody response to antigen leading to recurrent bacterial infections (Salzar et al. 2014).Alternatively spliced transcript variants encoding multiple isoforms have been observed for IL21 (provided by RefSeq, Dec 2011).

Rabbit anti Human interleukin-21 antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti interleukin-21 detects a band of approximately 17 kDa in NIH/3T3 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the C-terminal region of Human interleukin-21

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9HBE4
Entrez Gene
IL21
GO Terms
GO:0007165 signal transduction
GO:0005125 cytokine activity
GO:0005134 interleukin-2 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0032740 positive regulation of interleukin-17 production
GO:0034105 positive regulation of tissue remodeling
GO:0042102 positive regulation of T cell proliferation
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0048469 cell maturation
GO:0050729 positive regulation of inflammatory response
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of IL-21 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml