IL-1RA antibody

Rabbit anti Human Interleukin-1RA

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-1RA

Product Code Applications Pack Size List Price Your Price Qty
AHP2184
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SDS Safety Datasheet SDS
E FN WB 0.1 mg loader
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Rabbit anti Human interleukin-1 receptor antagonist antibody recognises human Interleukin-1 receptor antagonist (IL-1RA), a naturally occurring inflammatory inhibitor protein, produced by monocytes, macrophages, neutrophils, corneal epithelial cells and fibroblasts, which inhibits the activity of IL-1 alpha and IL-1 beta, by competitively blocking binding to cell surface receptors for IL-1.

Genetic variations in IL-1RA gene (IL1RN) are associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4), and IL-1RA has therapeutic applications in rheumatoid arthritis, asthma, psoriasis, sepsis, chronic myelogenous leukaemia and inflammatory bowel disease.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antiserum to human IL-1RA were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human IL-1RA (PHP080)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 2.5 3.7ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This purified human IL-1RA antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human IL-1RA antibody (AHP2184B) as the detection reagent and recombinant human IL-1RA (PHP080) as the standard.
Western Blotting
This purified human IL-1RA antibody may used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human IL-1RA (PHP080) as the positive control

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Synonyms
Interleukin-1 Receptor Antagonist
RRID
AB_10846099
UniProt
P18510
Entrez Gene
IL1RN
GO Terms
GO:0005886 plasma membrane
GO:0005125 cytokine activity
GO:0005152 interleukin-1 receptor antagonist activity
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005813 centrosome
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0051384 response to glucocorticoid stimulus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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