IL-1RA antibody
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Rabbit anti Human Interleukin-1RA
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-1RA
Antibody Quality and 
Unrivaled 
| Rabbit anti Human interleukin-1 receptor antagonist antibody recognises human Interleukin-1 receptor antagonist (IL-1RA), a naturally occurring inflammatory inhibitor protein, produced by monocytes, macrophages, neutrophils, corneal epithelial cells and fibroblasts, which inhibits the activity of IL-1 alpha and IL-1 beta, by competitively blocking binding to cell surface receptors for IL-1. Genetic variations in IL-1RA gene (IL1RN) are associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4), and IL-1RA has therapeutic applications in rheumatoid arthritis, asthma, psoriasis, sepsis, chronic myelogenous leukaemia and inflammatory bowel disease. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antiserum to human IL-1RA were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-1RA (PHP080)
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 6 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
2.5 | 3.7ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This purified human IL-1RA antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human IL-1RA antibody (AHP2184B) as the detection reagent and recombinant human IL-1RA (PHP080) as the standard.
- Western Blotting
- This purified human IL-1RA antibody may used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human IL-1RA (PHP080) as the positive control
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- Synonyms
- Interleukin-1 Receptor Antagonist
- RRID
- AB_10846099
- UniProt
- P18510
- Entrez Gene
- IL1RN
- GO Terms
- GO:0005886 plasma membrane
- GO:0005125 cytokine activity
- GO:0005152 interleukin-1 receptor antagonist activity
- GO:0005615 extracellular space
- GO:0005634 nucleus
- GO:0005813 centrosome
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0051384 response to glucocorticoid stimulus
- View More GO Terms
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