IL-12 antibody

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Goat anti Human Interleukin-12:Biotin

Goat anti Human Interleukin-12

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP779B E WB 50 µg
AHP779 E FN WB 0.1 mg
Rabbit anti Human Interleukin-12 antibody, recognizes human IL-12. IL-12 is a pleiotropic 75kDa heterodimeric glycoprotein, consisting of disulfide-linked IL-12A (p35) and IL-12B (p40) subunits, which signals through the high affinity IL-12 receptor.

IL-12 is produced primarily by antigen presenting cells and was originally called cytotoxic lymphocyte maturation factor (CLMF), identified by its ability to induce cytotoxic effector cells, in synergy with IL-2. IL-12 is an important factor in the T helper cell (Th1) immune response, promotes the development and activity of activated T cells and natural killer cells (NK), induces IFN-gamma production, and enhances resistance against invading pathogens.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. N.B For functional studies do not add azide.
Antiserum Preparation
Antisera to human IL-12 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant human IL-12 (PHP100)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P29459 Related reagents
P29460 Related reagents
Entrez Gene
IL12A Related reagents
IL12B Related reagents
GO Terms
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0002860 positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005143 interleukin-12 receptor binding
GO:0006955 immune response
GO:0007050 cell cycle arrest
GO:0008083 growth factor activity
GO:0050830 defense response to Gram-positive bacterium
GO:0010224 response to UV-B
GO:0016477 cell migration
GO:0032496 response to lipopolysaccharide
GO:0032700 negative regulation of interleukin-17 production
GO:0032729 positive regulation of interferon-gamma production
GO:0032816 positive regulation of natural killer cell activation
GO:0034393 positive regulation of smooth muscle cell apoptosis
GO:0042520 positive regulation of tyrosine phosphorylation of Stat4 protein
GO:0043514 interleukin-12 complex
GO:0045513 interleukin-27 binding
GO:0045785 positive regulation of cell adhesion
GO:0046982 protein heterodimerization activity
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0051135 positive regulation of NK T cell activation
GO:0002230 positive regulation of defense response to virus by host
GO:0002827 positive regulation of T-helper 1 type immune response
GO:0016020 membrane
GO:0004896 cytokine receptor activity
GO:0050829 defense response to Gram-negative bacterium
GO:0010535 positive regulation of activation of JAK2 kinase activity
GO:0019953 sexual reproduction
GO:0032693 negative regulation of interleukin-10 production
GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production
GO:0032733 positive regulation of interleukin-10 production
GO:0032735 positive regulation of interleukin-12 production
GO:0032740 positive regulation of interleukin-17 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0032819 positive regulation of natural killer cell proliferation
GO:0034105 positive regulation of tissue remodeling
GO:0042088 T-helper 1 type immune response
GO:0042093 T-helper cell differentiation
GO:0042095 interferon-gamma biosynthetic process
GO:0042104 positive regulation of activated T cell proliferation
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0042510 regulation of tyrosine phosphorylation of Stat1 protein
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043382 positive regulation of memory T cell differentiation
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045672 positive regulation of osteoclast differentiation
GO:0050729 positive regulation of inflammatory response
GO:0051142 positive regulation of NK T cell proliferation
GO:0070743 interleukin-23 complex
Regulatory
For research purposes only

Applications of IL-12 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.15 0.3ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0ug/ml
Functional Assays 0.012 0.02ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Anti human IL-12:biotin conjugate may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with anti human IL-12:purified (AHP779) as the capture reagent and recombinant human IL-12 (PHP100) as the standard.
ELISA
Anti human IL-12:purified may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with anti human IL-12:biotin conjugate (AHP779B) as the detection reagent and recombinant human IL-12 (PHP100) as the standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for IL-12 antibody

  1. Munari, F. et al. (2014) Cytokine BAFF released by Helicobacter pylori-infected macrophages triggers the Th17 response in human chronic gastritis.
    J Immunol. 193 (11): 5584-94.

Further Reading

  1. Gubler, U. et al. (1991) Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor.
    Proc Natl Acad Sci U S A. 88 (10): 4143-7.
  2. Rossol, S. et al. (1997) Interleukin-12 induction of Th1 cytokines is important for viral clearance in chronic hepatitis B.
    J Clin Invest. 99 (12): 3025-33.