IL-10 antibody

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Rabbit anti Human Interleukin-10

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP795 E FN WB 0.1 mg
Rabbit anti Human Interleukin-10 antibody recognizes human IL-10 (Interleukin-10), an 18.5kDa immunosuppressive cytokine originally known as cytokine synthesis inhibiting factor (CSIF), which specifically regulates immune-mediated inflammation and shares high sequence homology with murine IL-10 and the viral protein BCRF1.

IL-10, produced by macrophages, mast cells, T cells, activated B cells and keratinocytes, inhibits the secretion of a variety of pro-inflammatory cytokines including TNFalpha, IL-1, IL-2, IL-3 and IL-8 and is chemotactic for CD8+ T cells. The suppressive ability of IL-10 has become a focus area for the treatment of Crohn’s disease, but with conflicting results.

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water

Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide.
Antiserum Preparation
Antisera to human IL-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human IL-10
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P22301 Related reagents
Entrez Gene
IL10 Related reagents
GO Terms
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0002904 positive regulation of B cell apoptosis
GO:0005125 cytokine activity
GO:0005141 interleukin-10 receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007253 cytoplasmic sequestering of NF-kappaB
GO:0007267 cell-cell signaling
GO:0008083 growth factor activity
GO:0030183 B cell differentiation
GO:0030595 leukocyte chemotaxis
GO:0030889 negative regulation of B cell proliferation
GO:0032715 negative regulation of interleukin-6 production
GO:0032800 receptor biosynthetic process
GO:0042092 type 2 immune response
GO:0042100 B cell proliferation
GO:0042130 negative regulation of T cell proliferation
GO:0043193 positive regulation of gene-specific transcription
GO:0045191 regulation of isotype switching
GO:0045347 negative regulation of MHC class II biosynthetic process
GO:0045355 negative regulation of interferon-alpha biosynthetic process
GO:0050715 positive regulation of cytokine secretion
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
Regulatory
For research purposes only

Applications of IL-10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Functional Assays 4.0 6.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This purified human IL-10antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IL-10 antibody (AHP795B) as the detection reagent and recombinant human IL-10 (PHP047A) as the standard.
Western Blotting
This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IL-10 (PHP047A) as the positive control.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Positive Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Recombinant Human Interleukin-10 PHP047A 10 µg E FN WB

Product Specific References

References for IL-10 antibody

  1. Gesser, B. et al. (1997) Identification of functional domains on human interleukin 10.
    Proc Natl Acad Sci U S A. 94: 14620-14625.
  2. NaqviS, E.H. et al. (2017) A Prospective Study of Altered Inflammatory Response and Its Clinical Outcome following Laparoscopic and Open Cholecystectomy.
    Iran J Med Sci. 42 (4): 347-53.

Further Reading

  1. Tilg, H. et al. (2002) Treatment of Crohn’s disease with recombinant human interleukin 10 induces the proinflammatory cytokine interferon gamma.
    Gut. 50: 191-195.