Rabbit anti IKK (pThr23) antibody
recognizes IKK, also known as inhibitor of nuclear factor kappa-B kinase subunit alpha, conserved helix-loop-helix ubiquitous kinase, nuclear factor NF-kappa-B inhibitor kinase alpha or transcription factor 16, when phosphorylated at threonine 23.
IKK is a cytoplasmic and nuclear serine kinase playing an essential role in the NFκB signaling pathway. IKK is phosphorylated at threonine 23 by Akt, inducing its activity (Choi et al. 2016
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to IKK (pThr23) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding threonine 23 of human IKK
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
Toll signaling pathway
IkappaB kinase activity
identical protein binding
internal side of plasma membrane
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
CD40 receptor complex
positive regulation of I-kappaB kinase/NF-kappaB cascade
innate immune response
nerve growth factor receptor signaling pathway
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IKK (pThr23) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Choi, Y.J. et al. (2016) The underlying mechanism of proinflammatory NF-κB activation by the mTORC2/Akt/IKKα pathway during skin aging.
Oncotarget. 7 (33): 52685-94.
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