Igfbp-9 antibody

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Anti Human Igfbp-9 Antibody thumbnail image 2

Anti Human Igfbp-9 Antibody gallery image 1 — Formalin fixed, paraffin embedded human breast invasive carcinoma stained with Rabbit anti Human IGFBP-9 (**AHP1483**) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

Anti Human Igfbp-9 Antibody gallery image 2 — Formalin fixed, paraffin embedded human breast invasive carcinoma stained with Rabbit anti Human IGFBP-9 (**AHP1483**) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

Rabbit anti Human Igfbp-9

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1483	E P* WB			0.1 mg

Rabbit anti Human IGFBP-9 antibody recognizes human IGFBP-9 (insulin-like growth factor binding protein- 9), also known as NOV/CCN3, a 326 amino acid cysteine-rich secreted regulatory protein and member of the CCN family, containing the domains IGFBP, VWFC, C-Terminal cysteine knot-like and thrombospondin type I repeat.

IGFBP-9 is an inducer of cell migration and adhesion and shows increased expression in Wilm’s tumours and nephroblastomas. In contrast, decreased expression of IGFBP-9 correlates with an increase in tumourgenicity in certain cancers including childhood adrenocortical tumors (ACT).

Product Details

Target Species: Human
Product Form: Purified IgG - lyophilised
Reconstitution: Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation: Antiserum to human IGFBP-9 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Immunogen: Recombinant human IGFBP-9.
Approx. Protein Concentrations: IgG concentration 1.0mg/ml

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: P48745 Related reagents
Entrez Gene: NOV Related reagents
GO Terms: GO:0001558 regulation of cell growth; GO:0005520 insulin-like growth factor binding; GO:0008083 growth factor activity
Regulatory: For research purposes only

Applications of Igfbp-9 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.5	2.0ug/ml
Immunohistology - Paraffin ¹
Western Blotting	0.1	0.2ug/ml

¹ This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*

Product Specific References

References for Igfbp-9 antibody

Abd El Kader, T. et al. (2013) Anti-fibrotic effect of CCN3 accompanied by altered gene expression profile of the CCN family.
J Cell Commun Signal. 7 (1): 11-8.