IGFBP-7 antibody
Rabbit anti IGFBP-7
IGFBP-7 antibody recognizes insulin-like growth factor-binding protein 7, also known as IBP-7, IGF-binding protein 7, IGFBP-7, IGFBP-rP1, MAC25 protein, PGI2-stimulating factor, or TAF. The antibody has been extensively validated for western blotting using whole cell lysates.
IGFBP-7 Antibody with Control Lysate
IGFBP-7 antibody recognizes insulin-like growth factor-binding protein 7, also known as IBP-7 , IGF-binding protein 7 , IGFBP-7, IGFBP-rP1, MAC25 protein, PGI2-stimulating factor, prostacyclin-stimulating factor, tumor-derived adhesion factor or TAF.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00133 | WB | 100 µl | ||||
| VPA00133KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human IGFBP-7 antibody recognizes the insulin-like growth factor-binding protein 7, also known as IBP-7, IGF-binding protein 7, IGFBP-rP1, MAC25 protein, PGI2-stimulating factor, prostacyclin-stimulating factor, tumor-derived adhesion factor or TAF.
Encoded by IGFBP7, insulin-like growth factor-binding protein 7 is a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for IGFBP7, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307) (provided by RefSeq, Dec 2011).
Rabbit anti Human IGFBP-7 antibody detects a band of 29 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by IGFBP7, insulin-like growth factor-binding protein 7 is a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for IGFBP7, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307) (provided by RefSeq, Dec 2011).
Rabbit anti Human IGFBP-7 antibody detects a band of 29 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti IGFBP-7 detects a band of approximately 29 kDa in HeLa cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography.
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli derived recombinant human IGFBP-7
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q16270 Related reagents
- Entrez Gene
- IGFBP7 Related reagents
- GO Terms
- GO:0001558 regulation of cell growth
- GO:0007155 cell adhesion
- GO:0005520 insulin-like growth factor binding
- GO:0005615 extracellular space
- GO:0008285 negative regulation of cell proliferation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of IGFBP-7 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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