IGF2BP-3 antibody

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Rabbit anti IGF2BP-3

Rabbit anti human IGF2BP-3 antibody recognizes insulin-like growth factor 2 mRNA-binding protein 3, also known as IMP3, IGF-II mRNA-binding protein 3, VICKZ family member 3, VICKZ3, cancer/testis antigen 98 or CT98. This antibody has been extensively validated for western blotting.

IGF2BP-3 Antibody with Control Lysate

Rabbit anti human IGF2BP-3 antibody recognizes insulin-like growth factor 2 mRNA-binding protein 3, also known as IMP3, IGF-II mRNA-binding protein 3, KH domain containing protein overexpressed in cancer, VICKZ family member 3, VICKZ3, cancer/testis antigen 98 or CT98.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00385 WB 100 µl
VPA00385KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human IGF2BP-3 antibody recognizes insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP-3), also known as IMP3, KH domain containing protein overexpressed in cancer, VICKZ3 and CT98.

IGF2BP-3 is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. This protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes (provided by RefSeq, Jul 2008).

Rabbit anti Human IGF2BP-3 antibody detects a band of 69 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti IGF2BP-3 detects a band of approximately 69 kDa in K562 cell lysate
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide corresponding to the central region of human IGF2BP-3
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
O00425 Related reagents
Entrez Gene
IGF2BP3 Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0003730 mRNA 3'-UTR binding
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006412 translation
GO:0009653 anatomical structure morphogenesis
GO:0017148 negative regulation of translation
GO:0042035 regulation of cytokine biosynthetic process
GO:0045182 translation regulator activity
GO:0048027 mRNA 5'-UTR binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of IGF2BP-3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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