IGF2BP-3 antibody

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Rabbit anti IGF2BP-3

Rabbit anti human IGF2BP-3 antibody recognizes insulin-like growth factor 2 mRNA-binding protein 3, also known as IMP3, IGF-II mRNA-binding protein 3, VICKZ family member 3, VICKZ3, cancer/testis antigen 98 or CT98. This antibody has been extensively validated for western blotting.

IGF2BP-3 Antibody with Control Lysate

Rabbit anti human IGF2BP-3 antibody recognizes insulin-like growth factor 2 mRNA-binding protein 3, also known as IMP3, IGF-II mRNA-binding protein 3, KH domain containing protein overexpressed in cancer, VICKZ family member 3, VICKZ3, cancer/testis antigen 98 or CT98.

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00385	WB	100 µl
VPA00385KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Rabbit anti Human IGF2BP-3 antibody recognizes insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP-3), also known as IMP3, KH domain containing protein overexpressed in cancer, VICKZ3 and CT98.

IGF2BP-3 is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. This protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes (provided by RefSeq, Jul 2008).

Rabbit anti Human IGF2BP-3 antibody detects a band of 69 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti IGF2BP-3 detects a band of approximately 69 kDa in K562 cell lysate
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography
Preparation: 20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose
Immunogen: Synthetic peptide corresponding to the central region of human IGF2BP-3
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg K562 lysate lyophilized in RIPA buffer
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch
Shelf Life: As supplied, 12 months from date of despatch

More Information

UniProt: O00425 Related reagents
Entrez Gene: IGF2BP3 Related reagents
GO Terms: GO:0000166 nucleotide binding; GO:0005515 protein binding; GO:0003730 mRNA 3'-UTR binding; GO:0005829 cytosol; GO:0005634 nucleus; GO:0006412 translation; GO:0009653 anatomical structure morphogenesis; GO:0017148 negative regulation of translation; GO:0042035 regulation of cytokine biosynthetic process; GO:0045182 translation regulator activity; GO:0048027 mRNA 5'-UTR binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of IGF2BP-3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB

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