IGF-II antibody

100% Secure


Rabbit anti Human Insulin-Like Growth Factor II:Biotin

Rabbit anti Human Insulin-Like Growth Factor II

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP776B E WB 50 µg
AHP776 E FN P WB 0.1 mg
Rabbit anti Human Insulin-Like Growth Factor II antibody recognizes human IGF-II (Insulin-like Growth Factor II), a 67 amino acid mitogenic polypeptide and member of the Insulin gene family, synthesized by various foetal tissues during prenatal development, under the influence of placental lactogen.

IGF-II is a growth factor which exhibits both paracrine and autocrine properties and signals by binding to the type 1 and type 2 insulin-like growth factor receptors (type 1 IGFR and type 2 IGFR), and also to the insulin receptor (IR). The availability of IGF-II is dependent upon its attachment to, and release from, various IGF binding proteins (IGFBPs), a relationship which has been investigated in breast cancer research (Singer et al. 2004).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human IGF-II were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant human IGF-II
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P01344 Related reagents
Entrez Gene
IGF2 Related reagents
GO Terms
GO:0001501 skeletal system development
GO:0001503 ossification
GO:0005158 insulin receptor binding
GO:0005159 insulin-like growth factor receptor binding
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0006006 glucose metabolic process
GO:0006349 regulation of gene expression by genetic imprinting
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0032583 regulation of gene-specific transcription
GO:0043410 positive regulation of MAPKKK cascade
GO:0045840 positive regulation of mitosis
GO:0046628 positive regulation of insulin receptor signaling pathway
GO:0051781 positive regulation of cell division
GO:0051897 positive regulation of protein kinase B signaling cascade
Regulatory
For research purposes only

Applications of IGF-II antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0ug/ml
Functional Assays 3.1 4.6ug/ml
Immunohistology - Paraffin
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human IGF-II antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IGF-II antibody (AHP776) as the capture reagent and recombinant human IGF-II (PHP034A) as the standard.
ELISA
This purified human IGF-II antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IGF-II antibody (AHP776B) as the detection reagent and recombinant human IGF-II (PHP034A) as the standard.
Western Blotting
This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IGF-II (PHP034A) as the positive control.
Western Blotting
This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IGF-II (PHP034A) as the positive control.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Human Insulin-Like Growth Factor II AHP776 0.1 mg E FN P WB
Rabbit anti Human Insulin-Like Growth Factor II:Biotin AHP776B 50 µg E WB
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for IGF-II antibody

  1. Terasawa, H. et al. (1994) Solution structure of human insulin-like growth factor II; recognition sites for receptors and binding proteins.
    EMBO J. 13 (23): 5590-7.
  2. Singer, C.F. et al. (2004) Insulin-like growth factor (IGF)-I and IGF-II serum concentrations in patients with benign and malignant breast lesions: free IGF-II is correlated with breast cancer size.
    Clin Cancer Res. 10 (12 Pt 1): 4003-9.