IGF-II antibody
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P ResetRabbit anti Human Insulin-Like Growth Factor II
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IGF-II
Antibody Quality and Performance Guarantee
| Rabbit anti Human Insulin-Like Growth Factor II antibody recognizes human IGF-II (Insulin-like Growth Factor II), a 67 amino acid mitogenic polypeptide and member of the Insulin gene family, synthesized by various foetal tissues during prenatal development, under the influence of placental lactogen. IGF-II is a growth factor which exhibits both paracrine and autocrine properties and signals by binding to the type 1 and type 2 insulin-like growth factor receptors (type 1 IGFR and type 2 IGFR), and also to the insulin receptor (IR). The availability of IGF-II is dependent upon its attachment to, and release from, various IGF binding proteins (IGFBPs), a relationship which has been investigated in breast cancer research (Singer et al. 2004). |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human IGF-II were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IGF-II.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
3.1 | 4.6ug/ml |
| Immunohistology - Paraffin | |
||
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- This antibody may be used in Western Blotting under either reducing or non-reducing conditions.
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References for IGF-II antibody
-
Terasawa, H. et al. (1994) Solution structure of human insulin-like growth factor II; recognition sites for receptors and binding proteins.
EMBO J. 13 (23): 5590-7. -
Singer, C.F. et al. (2004) Insulin-like growth factor (IGF)-I and IGF-II serum concentrations in patients with benign and malignant breast lesions: free IGF-II is correlated with breast cancer size.
Clin Cancer Res. 10 (12 Pt 1): 4003-9.
- Synonyms
- Insulin-Like Growth Factor II
- RRID
- AB_2122535
- UniProt
- P01344
- Entrez Gene
- IGF2
- GO Terms
- GO:0001501 skeletal system development
- GO:0001503 ossification
- GO:0005158 insulin receptor binding
- GO:0005159 insulin-like growth factor receptor binding
- GO:0005179 hormone activity
- GO:0005615 extracellular space
- GO:0006006 glucose metabolic process
- GO:0006349 regulation of gene expression by genetic imprinting
- GO:0008083 growth factor activity
- View More GO Terms
- GO:0008284 positive regulation of cell proliferation
- GO:0008286 insulin receptor signaling pathway
- GO:0032583 regulation of gene-specific transcription
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045840 positive regulation of mitosis
- GO:0046628 positive regulation of insulin receptor signaling pathway
- GO:0051781 positive regulation of cell division
- GO:0051897 positive regulation of protein kinase B signaling cascade
AHP776
150818
153697
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