IFITM3 antibody
Rabbit anti IFITM3
Rabbit anti human IFITM3 antibody recognizes interferon-induced transmembrane protein 3, also known as Fragilis, dispanin subfamily A member 2b or interferon-inducible protein 1-8U. This antibody has been extensively validated for western blotting using whole cell lysates.
IFITM3 Antibody with Control Lysate
Rabbit anti human IFITM3 antibody recognizes interferon-induced transmembrane protein 3, also known as Fragilis, dispanin subfamily A member 2b or interferon-inducible protein 1-8U. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00312 | WB | 100 µl | ||||
| VPA00312KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human IFITM3 antibody recognizes the IFITM3, also known as fragilis, dispanin subfamily A member 2b and interferon-inducible protein 1-8U.
The protein encoded by the IFITM3 gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for IFITM3. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined (provided by RefSeq, May 2012).
Rabbit anti IFITM3 antibody detects a band of 15 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by the IFITM3 gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for IFITM3. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined (provided by RefSeq, May 2012).
Rabbit anti IFITM3 antibody detects a band of 15 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti IFITM3 detects a band of approximately 15 kDa in HeLa cell lysate
- Product Form
- Ig fraction - liquid
- Product Form
- Ig fraction - liquid
- Preparation
- Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Preparation
- 20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 1-30 of human IFITM3
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q01628 Related reagents
- Entrez Gene
- IFITM3 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0009615 response to virus
- GO:0016021 integral to membrane
- GO:0045087 innate immune response
- GO:0060337 type I interferon-mediated signaling pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of IFITM3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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