IFIH1 antibody

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Goat anti Human IFIH1 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2065 E P* 0.1 mg
Goat anti Human IFI1 antibody recognizes human Interferon-induced helicase C domain-containing protein 1, also known as IFIH1, Clinically amyopathic dermatomyositis autoantigen 140 kDa, CADM-140 autoantigen, Helicase with 2 CARD domains, Helicard, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, MDA-5, Murabutide down-regulated protein, RIG-I-like receptor 2, RLR-2 or RNA helicase-DEAD box protein 116.
Variations in the IFIH1 gene affect suceptibility to Insulin dependent diabetes mellitus 19 (IDDM19), a multifactorial disorder of glucose homeostasis with a susceptibility to ketoacidosis (Smyth et al. 2006). Mutations in the IFIH1 gene can lead to the development of Aicardi-Goutieres syndrome 7 (AGS7) a disease characterised by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection (Oda et al. 2014). Mutations in the IFIH1 gene may also lead to the development of Singleton-Merten syndrome 1 (SGMRT1), a disease featuring aortic calcification, dental abnormalities, osteopenia and acro-osteolysis (Rutsch et al. 2015).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human IFIH1 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence SNGYSTDENFRYL-C from the N-Terminal region of IFIH1 (NP_071451.2).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9BYX4 Related reagents
Entrez Gene
IFIH1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0009615 response to virus
GO:0004386 helicase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0008270 zinc ion binding
GO:0042981 regulation of apoptosis
GO:0032480 negative regulation of type I interferon production
GO:0043021 ribonucleoprotein binding
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of IFIH1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/32000
Immunohistology - Paraffin 1 10ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human pancreas

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P