iASPP antibody
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Rabbit anti Human iASPP
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- iASPP
Antibody Quality and Performance Guarantee
| Rabbit anti Human iASPP antibody detects human inhibitor of apoptosis stimulating protein of p53 (iASPP), which plays a central role in regulation of apoptosis and transcription via its interaction with NFkB and p53 proteins. At present, the iASPP gene is thought to encode three isoforms; isoform 1 is predominantly located in the cytoplasm, whilst isoforms 2 and 3 are nuclear proteins. iASPP is highly expressed in the heart, placenta and prostate. iASPP has been shown to have oncogenic properties in human breast carcinomas. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Dog Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human iASPP were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding amino acids 780-797 of human iASPP (isoform 1) protein.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/40000 | 1/160000 |
| Immunohistology - Paraffin | |
||
| Western Blotting | |
1/1000 | 1/5000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1338 detects a bands of approximately 100 and 50kDa corresponding to iASPP isoforms 1 and 2 respectively.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg | Log in | |||
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg | Log in | |||
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg | Log in | |||
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:FITC | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for iASPP antibody
-
Zhang, X. et al. (2007) Identification of a novel isoform of iASPP and its interaction with p53.
J Mol Biol. 368 (4): 1162-71.
- Synonyms
- Inhibitor Of Apoptosis Stimulating Protein Of p53
- RRID
- AB_2168575
- UniProt
- Q8WUF5
- Entrez Gene
- PPP1R13L
- GO Terms
- GO:0003714 transcription corepressor activity
- GO:0006915 apoptosis
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0008134 transcription factor binding
AHP1338
157696
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