iASPP antibody

Rabbit anti Human iASPP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
iASPP

Product Code Applications Pack Size List Price Your Price Qty
AHP1338
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SDS Safety Datasheet SDS
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Rabbit anti Human iASPP antibody detects human inhibitor of apoptosis stimulating protein of p53 (iASPP), which plays a central role in regulation of apoptosis and transcription via its interaction with NFkB and p53 proteins. At present, the iASPP gene is thought to encode three isoforms; isoform 1 is predominantly located in the cytoplasm, whilst isoforms 2 and 3 are nuclear proteins. iASPP is highly expressed in the heart, placenta and prostate.

iASPP has been shown to have oncogenic properties in human breast carcinomas.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Dog Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human iASPP were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding amino acids 780-797 of human iASPP (isoform 1) protein.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/40000 1/160000
Immunohistology - Paraffin
Western Blotting 1/1000 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1338 detects a bands of approximately 100 and 50kDa corresponding to iASPP isoforms 1 and 2 respectively.

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References for iASPP antibody

  1. Zhang, X. et al. (2007) Identification of a novel isoform of iASPP and its interaction with p53.
    J Mol Biol. 368 (4): 1162-71.

Synonyms
Inhibitor Of Apoptosis Stimulating Protein Of p53
RRID
AB_2168575
UniProt
Q8WUF5
Entrez Gene
PPP1R13L
GO Terms
GO:0003714 transcription corepressor activity
GO:0006915 apoptosis
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0008134 transcription factor binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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