HUWE1 antibody

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Rabbit anti HUWE1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2039 P WB 50 µg
Rabbit anti Human HUWE1 antibody recognizes human E3 ubiquitin-protein ligase HUWE1, also known as HUWE1, ARF-binding protein 1, ARF-BP1, HECT, UBA and WWE domain-containing protein 1, Homologous to E6AP carboxyl terminus homologous protein 9, HectH9, Large structure of UREB1, LASU1, Mcl-1 ubiquitin ligase E3, Mule or Upstream regulatory element-binding protein 1. HUWE1 is a 4374 amino acid DNA binding protein with a predicted molecular mass of ~480 kDa.

HUWE1 is an E3 ubiquitin-protein ligase involved in mediating ubiquitination and subsequent proteasomal degradation of target proteins. HUWE1 is also involved in apoptosis through catalyzation of the poly-ubiquitination and degradation of MCL1, ubiquitination of the p53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Research indicates that HUWE1 regulates the abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation (Hall et al. 2007).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human HUWE1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to an 19 amino acid sequence from near the carboxy terminal of human HUWE1.
Approx. Protein Concentrations
Total protein concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
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Entrez Gene
HUWE1 Related reagents
Regulatory
For research purposes only

Applications of HUWE1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 5ug/ml
Western Blotting 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Human brain (cortex) or human heart

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P