HTRA2 antibody

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Rabbit anti HTRA2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2707
Datasheet
WB
SDS
50 µg loader

Rabbit anti HtrA2 antibody recognizes protein serine protease HtrA2, also known as OMI and PRSS25. HtrA2 is a member of the HtrA serine protease family (Gray et al. 2000) and was identified as a mammalian homologue of the Escherishia coli protein HtrA (Faccio et al. 2000). HtrA2 forms a homotrimer which is important for its function and serine protease activity (Li et al. 2002). HtrA2 is released from mitochondria and interacts with X chromosome-linked inhibitor of apoptosis (XIAP) (Suzuki et al. 2001). The XIAP binding through a reaper-like motif is required for induction of apoptosis (Martins et al. 2002). HtrA2 is upregulated in hepatocellular carcinoma (Wang et al. 2018) and has been associated with chemosentistivity in various cancers (Miyamoto et al. 2015).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to HtrA2 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
0.42% Potassium phosphate
0.87% Sodium chloride
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
30% Glycerol
Immunogen
Recombinant human HtrA2

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O43464
Entrez Gene
HTRA2
GO Terms
GO:0051082 unfolded protein binding
GO:0004252 serine-type endopeptidase activity
GO:0006915 apoptosis
GO:0005634 nucleus
GO:0005758 mitochondrial intermembrane space
GO:0005789 endoplasmic reticulum membrane
GO:0006508 proteolysis
GO:0006950 response to stress
GO:0009898 internal side of plasma membrane
GO:0031966 mitochondrial membrane
GO:0035631 CD40 receptor complex
Regulatory
For research purposes only

Applications of HTRA2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti HtrA2 antibody detects a band of approximately 38 kDa in HeLa, MCF7 cell lysates and mouse heart and mouse kidney tissue lysates

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg