Rabbit anti HtrA2 antibody
recognizes protein serine protease HtrA2, also known as OMI and PRSS25. HtrA2 is a member of the HtrA serine protease family (Gray et al. 2000
) and was identified as a mammalian homologue of the Escherishia coli
protein HtrA (Faccio et al. 2000
). HtrA2 forms a homotrimer which is important for its function and serine protease activity (Li et al. 2002
). HtrA2 is released from mitochondria and interacts with X chromosome-linked inhibitor of apoptosis (XIAP) (Suzuki et al. 2001
). The XIAP binding through a reaper-like motif is required for induction of apoptosis (Martins et al. 2002
). HtrA2 is upregulated in hepatocellular carcinoma (Wang et al. 2018
) and has been associated with chemosentistivity in various cancers (Miyamoto et al. 2015
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to HtrA2 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- 0.42% Potassium phosphate
0.87% Sodium chloride
- Preservative Stabilisers
- 0.01% Sodium Azide (NaN3)
- Recombinant human HtrA2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
unfolded protein binding
serine-type endopeptidase activity
mitochondrial intermembrane space
endoplasmic reticulum membrane
response to stress
internal side of plasma membrane
CD40 receptor complex
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of HTRA2 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti HtrA2 antibody detects a band of approximately 38 kDa in HeLa, MCF7 cell lysates and mouse heart and mouse kidney tissue lysates
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Secondary Antibodies Available
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