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Hsc70 antibody

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Anti Hsc70 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1
Anti Hsc70 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Rabbit anti Hsc70 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to Hsc70 (molecular weight 75 kDa)

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WB

Rabbit anti Hsc70

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Hsc70

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Product Code Applications Pack Size List Price Your Price Qty
VPA00276
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl
List Price Your Price

Rabbit anti Human Hsc70 antibody recognizes Hsc70 also known as HSPA8, HSC71, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, epididymis luminal protein 33, epididymis secretory sperm binding protein Li 72p, heat shock 70kd protein 10, heat shock cognate protein 54 and lipopolysaccharide-associated protein 1.

The Hsc70 gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for Hsc70 (provided by RefSeq, Aug 2011).

Rabbit anti Human Hsc70 protein antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti Hsc70 detects a band of approximately 75 kDa in HEK293 cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 82-110 of human Hsc70
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml
List Price Your Price
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
P11142
Entrez Gene
HSPA8
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0030529 ribonucleoprotein complex
GO:0006457 protein folding
GO:0042623 ATPase activity, coupled
GO:0006986 response to unfolded protein
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006892 post-Golgi vesicle-mediated transport
GO:0007269 neurotransmitter secretion
GO:0009986 cell surface
GO:0016044 cellular membrane organization
GO:0016071 mRNA metabolic process
GO:0042470 melanosome
GO:0044419 interspecies interaction between organisms
GO:0061202 clathrin sculpted gamma-aminobutyric acid transport vesicle membrane

VPA00276

160415 160425

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