Hsc70 antibody

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Rabbit anti Hsc70

Rabbit anti human Hsc70 antibody recognizes heat shock cognate 71 kDa protein, also known as HSPA8, HSC71, LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, or lipopolysaccharide-associated protein 1. This antibody has been extensively validated for western blotting.

Hsc70 Antibody with Control Lysate

Rabbit anti human Hsc70 antibody recognizes heat shock cognate 71 kDa protein, also known as HSPA8, HSC71, LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, epididymis luminal protein 33, or lipopolysaccharide-associated protein 1.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00276 WB 100 µl
VPA00276KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human Hsc70 antibody recognizes Hsc70 also known as HSPA8, HSC71, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, epididymis luminal protein 33, epididymis secretory sperm binding protein Li 72p, heat shock 70kd protein 10, heat shock cognate protein 54 and lipopolysaccharide-associated protein 1.

The Hsc70 gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for Hsc70 (provided by RefSeq, Aug 2011).

Rabbit anti Human Hsc70 protein antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti Hsc70 detects a band of approximately 75 kDa in HEK293 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Preparation
20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 82-110 of human Hsc70
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P11142 Related reagents
Entrez Gene
HSPA8 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0030529 ribonucleoprotein complex
GO:0006457 protein folding
GO:0042623 ATPase activity, coupled
GO:0006986 response to unfolded protein
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006892 post-Golgi vesicle-mediated transport
GO:0007269 neurotransmitter secretion
GO:0009986 cell surface
GO:0016044 cellular membrane organization
GO:0016071 mRNA metabolic process
GO:0042470 melanosome
GO:0044419 interspecies interaction between organisms
GO:0061202 clathrin sculpted gamma-aminobutyric acid transport vesicle membrane
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Hsc70 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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