Hsc70 antibody

100% Secure


Goat anti Human Hsc70

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1571 E WB 0.1 mg
Goat anti Human Hsc70 antibody recognizes human Heat shock cognate 71 kDa protein, also known as Heat shock 70 kDa protein 8 or Lipopolysaccharide-associated protein 1. Hsc70 is a 646 amino acid ubiquitously expressed cytoplasmic heat-shock cognate protein and member of the heat shock protein 70 family, encoded for by the HSPA8 gene. Goat anti Human Hsc70 antibody recognizes epitopes in the region between amino acids 555 and 570 of Hsc70 isoform 1, missing in isoform 2.

Heat shock proteins are a group of constitutively expressed or heat/stress-inducible proteins which act as molecular chaperones and play an essential role in protein folding/unfolding and transport of proteins across membranes. Hsc70 facilitates the correct folding of newly-synthesized polypeptides (Flynn et al. 1989) and also participates in the ATPase disassociation of clathrin-coated vesicles during endocytosis, in conjunction with the co-chaperone auxilin (Jiang et al. 1997).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Rat Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human Hsc70 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Tris buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-EKLQGKINDEDKQK corresponding to the internal region of Hsc70 (NP_006588.1)
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P11142 Related reagents
Entrez Gene
HSPA8 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0030529 ribonucleoprotein complex
GO:0006457 protein folding
GO:0042623 ATPase activity, coupled
GO:0006986 response to unfolded protein
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006892 post-Golgi vesicle-mediated transport
GO:0007269 neurotransmitter secretion
GO:0009986 cell surface
GO:0016044 cellular membrane organization
GO:0016071 mRNA metabolic process
GO:0042470 melanosome
GO:0044419 interspecies interaction between organisms
GO:0061202 clathrin sculpted gamma-aminobutyric acid transport vesicle membrane
Regulatory
For research purposes only

Applications of Hsc70 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/4000
Western Blotting 1.0 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
This product detects a band of approximately 75kDa in A431 cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

Further Reading

  1. Schmid, S.L. (1997) Clathrin-coated vesicle formation and protein sorting: an integrated process.
    Annu Rev Biochem. 66: 511-48.