HR23B antibody

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Anti HR23B Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Goat anti Human HR23B antibody (**VPA00151**) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 28 second exposure. Arrow points to HR23B (molecular weight 58 kDa).

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Goat anti HR23B

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: HR23B

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Goat anti Human HR23B antibody recognizes HR23B also known as hHR23B and XP-C repair-complementing complex 58 kDa protein (p58).

HR23B is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. HR23B has also been shown to interact with and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. It contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms (provided by RefSeq, Sep 2011).

Goat anti Human HR23B antibody detects a band of 58 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti HR23B detects a band of approximately 58 kDa in LNCaP cell lysates.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Synthetic peptide corresponding to amino acids 163-176 of human HR23B
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt: P54727
Entrez Gene: RAD23B
GO Terms: GO:0000502 proteasome complex; GO:0000715 nucleotide-excision repair, DNA damage recognition; GO:0000718 nucleotide-excision repair, DNA damage removal; GO:0003684 damaged DNA binding; GO:0003697 single-stranded DNA binding; GO:0005737 cytoplasm; GO:0005654 nucleoplasm; GO:0031593 polyubiquitin binding; GO:0032434 regulation of proteasomal ubiquitin-dependent protein catabolic process; View More GO Terms; GO:0043161 proteasomal ubiquitin-dependent protein catabolic process; GO:0071942 XPC complex