HR23B antibody

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Goat anti HR23B

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00151
Datasheet
WB
MSDS
100 µl loader

Goat anti Human HR23B antibody recognizes HR23B also known as hHR23B and XP-C repair-complementing complex 58 kDa protein (p58).

HR23B is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. HR23B has also been shown to interact with and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. It contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms (provided by RefSeq, Sep 2011).

Goat anti Human HR23B antibody detects a band of 58 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti HR23B detects a band of approximately 58 kDa in LNCaP cell lysates.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 163-176 of human HR23B

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P54727
Entrez Gene
RAD23B
GO Terms
GO:0000502 proteasome complex
GO:0000715 nucleotide-excision repair, DNA damage recognition
GO:0000718 nucleotide-excision repair, DNA damage removal
GO:0003684 damaged DNA binding
GO:0003697 single-stranded DNA binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0031593 polyubiquitin binding
GO:0032434 regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0071942 XPC complex
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of HR23B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader