HR23B antibody

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Goat anti HR23B

HR23B antibody recognizes UV excision repair protein RAD23 homolog B, also known as RAD23, yeast homolog of B or XP-C repair complementing complex 58 kDa. This antibody has been extensively validated for western blotting using whole cell lysates.

HR23B Antibody with Control Lysate

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00151	WB	100 µl
VPA00151KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Goat anti Human HR23B antibody recognizes HR23B also known as hHR23B and XP-C repair-complementing complex 58 kDa protein (p58).

HR23B is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. HR23B has also been shown to interact with and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. It contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms (provided by RefSeq, Sep 2011).

Goat anti Human HR23B antibody detects a band of 58 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti HR23B detects a band of approximately 58 kDa in LNCaP cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography
Preparation: 20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Synthetic peptide corresponding to amino acids 163-176 of human HR23B
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg LNCaP lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P54727 Related reagents
Entrez Gene: RAD23B Related reagents
GO Terms: GO:0000502 proteasome complex; GO:0000715 nucleotide-excision repair, DNA damage recognition; GO:0000718 nucleotide-excision repair, DNA damage removal; GO:0003684 damaged DNA binding; GO:0003697 single-stranded DNA binding; GO:0005737 cytoplasm; GO:0005654 nucleoplasm; GO:0031593 polyubiquitin binding; GO:0032434 regulation of proteasomal ubiquitin-dependent protein catabolic process; GO:0043161 proteasomal ubiquitin-dependent protein catabolic process; GO:0071942 XPC complex
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of HR23B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C E P WB
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C E P WB

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