hnRNP K antibody
The hnRNP K protein belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. Encoded by the HNRNPK gene, hnRNP K is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for HNRNPK, however, not all of them are fully characterized (provided by RefSeq, Jul 2008).
Rabbit anti Human hnRNP K antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti hnRNP K detects a band of approximately 62 kDa in Raji cell lysates
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human hnRNP K
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0005515 protein binding
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0003677 DNA binding
- GO:0003723 RNA binding
- GO:0007165 signal transduction
- GO:0005737 cytoplasm
- GO:0005654 nucleoplasm
- GO:0005681 spliceosomal complex
- GO:0030530 heterogeneous nuclear ribonucleoprotein complex
- GO:0044419 interspecies interaction between organisms
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of hnRNP K antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Rabbit IgG (H/L):HRP||STAR208P||WB||2 ml|