hnRNP D antibody

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Anti hnRNP D Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with hnRNP D antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to hnRNP D (molecular weight 37-48 kDa).

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Rabbit anti hnRNP D

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: hnRNP D

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Rabbit anti Human hnRNP D antibody recognizes hnRNP D also known as heterogeneous nuclear ribonucleoprotein D0, ARE-binding protein AUFI, type A or AU-rich element RNA-binding protein 1.

hnRNP D belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by HNRNPD gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of HNRNPD results in four transcript variants (provided by RefSeq, Jul 2008).

Rabbit anti Human hnRNP D antibody detects a band of 37-48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti hnRNP D detects a band of approximately 37-48 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody prepared by affinity chromatography on Protein A.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose.
Immunogen: Synthetic peptide directed towards the C terminal region of human hnRNP D
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.