Histone Deacetylase 6 antibody

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Rabbit anti Histone Deacetylase 6

Histone deacetylase 6 antibody recognizes histone deacetylase 6, also known as protein phosphatase 1 or regulatory subunit 90. This antibody has been extensively validated for western blotting using whole cell lysates.

Histone Deacetylase 6 Antibody with Control Lysate

Histone deacetylase 6 antibody recognizes histone deacetylase 6, also known as protein phosphatase 1 or regulatory subunit 90. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00171 WB 100 µl
VPA00171KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human histone deacetylase 6 antibody recognizes histone deacetylase 6, also known as HDAC6.

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Encoded by HDAC6 gene, histone deacetylase 6 belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription (provided by RefSeq, Jul 2008).

Rabbit anti Human histone deacetylase 6 antibody detects a band of 160 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti histone deacetylase 6 detects a band of approximately 160 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<30% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<30% Glycerol
Immunogen
Synthetic peptide corresponding to amino acids close to the N terminus of human histone deacetylase 6
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9UBN7 Related reagents
Entrez Gene
HDAC6 Related reagents
GO Terms
GO:0000118 histone deacetylase complex
GO:0003779 actin binding
GO:0004407 histone deacetylase activity
GO:0005829 cytosol
GO:0005875 microtubule associated complex
GO:0005901 caveola
GO:0006350 transcription
GO:0006515 misfolded or incompletely synthesized protein catabolic process
GO:0008013 beta-catenin binding
GO:0008017 microtubule binding
GO:0008270 zinc ion binding
GO:0009636 response to toxin
GO:0009967 positive regulation of signal transduction
GO:0010469 regulation of receptor activity
GO:0051879 Hsp90 protein binding
GO:0010634 positive regulation of epithelial cell migration
GO:0010727 negative regulation of hydrogen peroxide metabolic process
GO:0010870 positive regulation of receptor biosynthetic process
GO:0016235 aggresome
GO:0016236 macroautophagy
GO:0016566 specific transcriptional repressor activity
GO:0016575 histone deacetylation
GO:0031252 cell leading edge
GO:0031593 polyubiquitin binding
GO:0032418 lysosome localization
GO:0034983 peptidyl-lysine deacetylation
GO:0042826 histone deacetylase binding
GO:0042903 tubulin deacetylase activity
GO:0043014 alpha-tubulin binding
GO:0043065 positive regulation of apoptosis
GO:0045861 negative regulation of proteolysis
GO:0048156 tau protein binding
GO:0048471 perinuclear region of cytoplasm
GO:0051354 negative regulation of oxidoreductase activity
GO:0060632 regulation of microtubule-based movement
GO:0060765 regulation of androgen receptor signaling pathway
GO:0070301 cellular response to hydrogen peroxide
GO:0070840 dynein complex binding
GO:0070842 aggresome assembly
GO:0070845 polyubiquitinated misfolded protein transport
GO:0070846 Hsp90 deacetylation
GO:0070848 response to growth factor stimulus
GO:0090035 positive regulation of cellular chaperone-mediated protein complex assembly
GO:0090042 tubulin deacetylation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Histone Deacetylase 6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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