Rabbit anti Histone deacetylase 5 (pSer498) antibody
recognizes histone deacetylase 5, also known as HDAC5, when phosphorylated at serine 498. Histone deacetylases are chromatin-modifying enzymes that are involved in regulation of many aspects of cell biology including tissue differentiation, autophagy, apoptosis, migration, mitosis and angiogenesis. Histone deacetylase 5 is a class II histone deacetylase, which shuttles back and forth between nucleus and cytoplasm.
Histone deacetylase 5 is phosphorylated at serine 498 by Protein kinase D (PKD) and Calcium/ calmodulin-dependent protein kinase (CaMK) (McKinsey et al. 2000
, Ha et al. 2008
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to histone deacetylase 5 (pSer498) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 498 of human histone deacetylase 5
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
histone deacetylase complex
histone deacetylase activity
protein kinase C binding
positive regulation of gene-specific transcription from RNA polymerase II promoter
negative regulation of gene-specific transcription from RNA polymerase II promoter
negative regulation of myotube differentiation
specific transcriptional repressor activity
B cell differentiation
cellular response to insulin stimulus
regulation of protein binding
positive regulation of transcription factor activity
repressing transcription factor binding
negative regulation of cell migration involved in sprouting angiogenesis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Histone Deacetylase 5 (pSer498) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
McKinsey, T.A. et al. (2000) Signal-dependent nuclear export of a histone deacetylase regulates muscle differentiation.
Nature. 408 (6808): 106-11.
Ha, C.H. et al. (2008) Protein kinase D-dependent phosphorylation and nuclear export of histone deacetylase 5 mediates vascular endothelial growth factor-induced gene expression and angiogenesis.
J Biol Chem. 283 (21): 14590-9.