Histone Deacetylase 5 antibody

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Rabbit anti Histone Deacetylase 5

Histone deacetylase 5 (HDAC 5) antibody recognizes histone deacetylase 5, also known as antigen NY-CO-9. This antibody has been extensively validated for western blotting using whole cell lysates.

Histone Deacetylase 5 Antibody with Control Lysate

Histone deacetylase 5 (HDAC 5) antibody recognizes histone deacetylase 5, also known as antigen NY-CO-9. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00173 WB 100 µl
VPA00173KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human histone deacetylase 5 antibody recognizes histone deacetylase 5, also known as HDAC5 and antigen NY-CO-9.

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. HDAC5 is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for HDAC5 (provided by RefSeq, Jul 2008).

Rabbit anti Human histone deacetylase 5 antibody detects a band of 124 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti histone deacetylase 5 detects a band of approximately 124 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
<50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
<50% Glycerol
Immunogen
Synthetic peptide corresponding to the internal region surrounding amino acid 661 of human histone deacetylase 5
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9UQL6 Related reagents
Entrez Gene
HDAC5 Related reagents
GO Terms
GO:0000118 histone deacetylase complex
GO:0004407 histone deacetylase activity
GO:0005080 protein kinase C binding
GO:0005737 cytoplasm
GO:0006338 chromatin remodeling
GO:0006342 chromatin silencing
GO:0006350 transcription
GO:0006954 inflammatory response
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010832 negative regulation of myotube differentiation
GO:0016566 specific transcriptional repressor activity
GO:0016575 histone deacetylation
GO:0030183 B cell differentiation
GO:0032869 cellular response to insulin stimulus
GO:0043393 regulation of protein binding
GO:0051091 positive regulation of transcription factor activity
GO:0070491 repressing transcription factor binding
GO:0090051 negative regulation of cell migration involved in sprouting angiogenesis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Histone Deacetylase 5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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