Histone Deacetylase 5 antibody

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Rabbit anti Histone Deacetylase 5

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2478 P WB 50 µl
Rabbit anti histone deacetylase 5 antibody recognizes histone deacetylase 5, also known as HDAC5. Histone deacetylases are chromatin-modifying enzymes that are involved in regulation of many aspects of cell biology including tissue differentiation, autophagy, apoptosis, migration, mitosis and angiogenesis. HDAC5 is a member of class IIa of the histone deacetylases which unlike class I is not found in the only in the nucleus but rather shuttles back and forth between nucleus and cytoplasm.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to histone deacetylase 5 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Synthetic human histone deacetylase 5

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q9UQL6 Related reagents
Entrez Gene
HDAC5 Related reagents
GO Terms
GO:0000118 histone deacetylase complex
GO:0004407 histone deacetylase activity
GO:0005080 protein kinase C binding
GO:0005737 cytoplasm
GO:0006338 chromatin remodeling
GO:0006342 chromatin silencing
GO:0006350 transcription
GO:0006954 inflammatory response
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010832 negative regulation of myotube differentiation
GO:0016566 specific transcriptional repressor activity
GO:0016575 histone deacetylation
GO:0030183 B cell differentiation
GO:0032869 cellular response to insulin stimulus
GO:0043393 regulation of protein binding
GO:0051091 positive regulation of transcription factor activity
GO:0070491 repressing transcription factor binding
GO:0090051 negative regulation of cell migration involved in sprouting angiogenesis
Regulatory
For research purposes only

Applications of Histone Deacetylase 5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/200
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti histone deacetylase 5 antibody detects a band of approximately 122 kDa in cell lysates under reducing conditions

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*