Rabbit anti histone deacetylase 5 antibody recognizes histone deacetylase 5, also known as HDAC5. Histone deacetylases are chromatin-modifying enzymes that are involved in regulation of many aspects of cell biology including tissue differentiation, autophagy, apoptosis, migration, mitosis and angiogenesis. HDAC5 is a member of class IIa of the histone deacetylases which unlike class I is not found in the only in the nucleus but rather shuttles back and forth between nucleus and cytoplasm.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to histone deacetylase 5 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Synthetic human histone deacetylase 5
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
histone deacetylase complex
histone deacetylase activity
protein kinase C binding
positive regulation of gene-specific transcription from RNA polymerase II promoter
negative regulation of gene-specific transcription from RNA polymerase II promoter
negative regulation of myotube differentiation
specific transcriptional repressor activity
B cell differentiation
cellular response to insulin stimulus
regulation of protein binding
positive regulation of transcription factor activity
repressing transcription factor binding
negative regulation of cell migration involved in sprouting angiogenesis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Histone Deacetylase 5 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti histone deacetylase 5 antibody detects a band of approximately 122 kDa in cell lysates under reducing conditions
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Secondary Antibodies Available
Useful Reagents Available
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