Histone Deacetylase 4 (pSer632) antibody

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Rabbit anti Histone Deacetylase 4 (pSer632)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP2632	WB			50 µl

Rabbit anti Histone deacetylase 4 (pSer632) antibody recognizes histone deacetylase 4, also known as HDAC4, when phosphorylated at serine 632. Histone deacetylases are chromatin-modifying enzymes that are involved in the regulation of many aspects of cell biology including tissue differentiation, autophagy, apoptosis, migration, mitosis and angiogenesis. Histone deacetylase 4 is a class II histone deacetylase, which shuttles back and forth between nucleus and cytoplasm.

Histone deacetylase 4 is phosphorylated at serine 632 by calcium/calmodulin-dependent protein kinase (CaMK) (Wang et al. 2014).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse	Expected from Sequence
Rat	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to histone deacetylase 4 (pSer632) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02% Sodium Azide
50% Glycerol

Immunogen

Phospho specific-peptide corresponding to residues surrounding serine 632 of human histone deacetylase 4

Storage Information

Storage: Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch

More Information

UniProt: P56524 Related reagents
Entrez Gene: HDAC4 Related reagents
GO Terms: GO:0000118 histone deacetylase complex; GO:0004407 histone deacetylase activity; GO:0006338 chromatin remodeling; GO:0006954 inflammatory response; GO:0007399 nervous system development; GO:0008270 zinc ion binding; GO:0008284 positive regulation of cell proliferation; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0010832 negative regulation of myotube differentiation; GO:0014894 response to denervation involved in regulation of muscle adaptation; GO:0014898 cardiac muscle hypertrophy in response to stress; GO:0016563 transcription activator activity; GO:0016566 specific transcriptional repressor activity; GO:0017053 transcriptional repressor complex; GO:0030183 B cell differentiation; GO:0030955 potassium ion binding; GO:0033235 positive regulation of protein sumoylation; GO:0033613 activating transcription factor binding; GO:0034983 peptidyl-lysine deacetylation; GO:0042826 histone deacetylase binding; GO:0043393 regulation of protein binding; GO:0043433 negative regulation of transcription factor activity; GO:0045820 negative regulation of glycolysis; GO:0051091 positive regulation of transcription factor activity; GO:0070491 repressing transcription factor binding; GO:0070555 response to interleukin-1; GO:0070932 histone H3 deacetylation; GO:0070933 histone H4 deacetylation
Regulatory: For research purposes only

Applications of Histone Deacetylase 4 (pSer632) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
TidyBlot™ Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*