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Histone Deacetylase 4 (pSer632) antibody

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Anti Histone Deacetylase 4 (pSer632) Antibody gallery image 1
Western blot analysis of extracts from HEK-293 cells untreated or treated with EGF using Rabbit anti histone deacetylase 4 (pSer632) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes

Rabbit anti Histone Deacetylase 4 (pSer632)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2632
Datasheet
WB
MSDS
50 µl loader

Rabbit anti Histone deacetylase 4 (pSer632) antibody recognizes histone deacetylase 4, also known as HDAC4, when phosphorylated at serine 632. Histone deacetylases are chromatin-modifying enzymes that are involved in the regulation of many aspects of cell biology including tissue differentiation, autophagy, apoptosis, migration, mitosis and angiogenesis. Histone deacetylase 4 is a class II histone deacetylase, which shuttles back and forth between nucleus and cytoplasm.

Histone deacetylase 4 is phosphorylated at serine 632 by calcium/calmodulin-dependent protein kinase (CaMK) (Wang et al. 2014).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to histone deacetylase 4 (pSer632) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 632 of human histone deacetylase 4

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P56524
Entrez Gene
HDAC4
GO Terms
GO:0000118 histone deacetylase complex
GO:0004407 histone deacetylase activity
GO:0006338 chromatin remodeling
GO:0006954 inflammatory response
GO:0007399 nervous system development
GO:0008270 zinc ion binding
GO:0008284 positive regulation of cell proliferation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010832 negative regulation of myotube differentiation
GO:0014894 response to denervation involved in regulation of muscle adaptation
GO:0014898 cardiac muscle hypertrophy in response to stress
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0017053 transcriptional repressor complex
GO:0030183 B cell differentiation
GO:0030955 potassium ion binding
GO:0033235 positive regulation of protein sumoylation
GO:0033613 activating transcription factor binding
GO:0034983 peptidyl-lysine deacetylation
GO:0042826 histone deacetylase binding
GO:0043393 regulation of protein binding
GO:0043433 negative regulation of transcription factor activity
GO:0045820 negative regulation of glycolysis
GO:0051091 positive regulation of transcription factor activity
GO:0070491 repressing transcription factor binding
GO:0070555 response to interleukin-1
GO:0070932 histone H3 deacetylation
GO:0070933 histone H4 deacetylation
Regulatory
For research purposes only

Applications of Histone Deacetylase 4 (pSer632) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

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Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Wang, Z. et al. (2014) HDAC4: mechanism of regulation and biological functions.
    Epigenomics. 6 (1): 139-50.
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