Histone Deacetylase 4 (pSer632) antibody
Rabbit anti Histone deacetylase 4 (pSer632) antibody recognizes histone deacetylase 4, also known as HDAC4, when phosphorylated at serine 632. Histone deacetylases are chromatin-modifying enzymes that are involved in the regulation of many aspects of cell biology including tissue differentiation, autophagy, apoptosis, migration, mitosis and angiogenesis. Histone deacetylase 4 is a class II histone deacetylase, which shuttles back and forth between nucleus and cytoplasm.
Histone deacetylase 4 is phosphorylated at serine 632 by calcium/calmodulin-dependent protein kinase (CaMK) (Wang et al. 2014).
Histone deacetylase 4 is phosphorylated at serine 632 by calcium/calmodulin-dependent protein kinase (CaMK) (Wang et al. 2014).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to histone deacetylase 4 (pSer632) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 632 of human histone deacetylase 4
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P56524
- Entrez Gene
- HDAC4
- GO Terms
- GO:0000118 histone deacetylase complex
- GO:0004407 histone deacetylase activity
- GO:0006338 chromatin remodeling
- GO:0006954 inflammatory response
- GO:0007399 nervous system development
- GO:0008270 zinc ion binding
- GO:0008284 positive regulation of cell proliferation
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010832 negative regulation of myotube differentiation
- GO:0014894 response to denervation involved in regulation of muscle adaptation
- GO:0014898 cardiac muscle hypertrophy in response to stress
- GO:0016563 transcription activator activity
- GO:0016566 specific transcriptional repressor activity
- GO:0017053 transcriptional repressor complex
- GO:0030183 B cell differentiation
- GO:0030955 potassium ion binding
- GO:0033235 positive regulation of protein sumoylation
- GO:0033613 activating transcription factor binding
- GO:0034983 peptidyl-lysine deacetylation
- GO:0042826 histone deacetylase binding
- GO:0043393 regulation of protein binding
- GO:0043433 negative regulation of transcription factor activity
- GO:0045820 negative regulation of glycolysis
- GO:0051091 positive regulation of transcription factor activity
- GO:0070491 repressing transcription factor binding
- GO:0070555 response to interleukin-1
- GO:0070932 histone H3 deacetylation
- GO:0070933 histone H4 deacetylation
- Regulatory
- For research purposes only
Applications of Histone Deacetylase 4 (pSer632) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Wang, Z. et al. (2014) HDAC4: mechanism of regulation and biological functions.
Epigenomics. 6 (1): 139-50.
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