Histone Deacetylase 3 antibody

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Rabbit anti Histone Deacetylase 3

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00557
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human histone deacetylase 3 antibody recognizes histone deacetylase 3 (HDAC3), also known as SMAP45 or RPD3-2.

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC3 gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. HDAC3 is regarded as a potential tumor suppressor gene (provided by RefSeq, Jul 2008).

Rabbit anti Human histone deacetylase 3 antibody detects a band of 49 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti histone deacetylase 3 detects a band of approximately 49 kDa in NIH/3T3 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 383-412 of human histone deacetylase 3

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
O15379
Entrez Gene
HDAC3
GO Terms
GO:0000118 histone deacetylase complex
GO:0003714 transcription corepressor activity
GO:0004407 histone deacetylase activity
GO:0005737 cytoplasm
GO:0005876 spindle microtubule
GO:0006916 anti-apoptosis
GO:0008134 transcription factor binding
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0016564 transcription repressor activity
GO:0017053 transcriptional repressor complex
GO:0042826 histone deacetylase binding
GO:0044255 cellular lipid metabolic process
GO:0045786 negative regulation of cell cycle
GO:0046329 negative regulation of JNK cascade
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051225 spindle assembly
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of Histone Deacetylase 3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml