Histone Deacetylase 3 antibody

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Rabbit anti Histone Deacetylase 3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2476 Ch IF IP P WB 50 µl
Rabbit anti histone deacetylase 3 antibody recognizes histone deacetylase 3 also known as HDAC3. Histone deacetylases are chromatin-modifying enzymes that are involved in regulation of many aspects of cell biology including tissue differentiation, autophagy, apoptosis, migration, mitosis and angiogenesis. Class I HDACs, which include HDAC1, 2, 3 and 8 are ubiquitously expressed in all tissues and have predominantly nuclear localization.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to histone deacetylase 3 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant human histone deacetylase 3

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
O15379 Related reagents
Entrez Gene
HDAC3 Related reagents
GO Terms
GO:0000118 histone deacetylase complex
GO:0003714 transcription corepressor activity
GO:0004407 histone deacetylase activity
GO:0005737 cytoplasm
GO:0005876 spindle microtubule
GO:0006916 anti-apoptosis
GO:0008134 transcription factor binding
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0016564 transcription repressor activity
GO:0017053 transcriptional repressor complex
GO:0042826 histone deacetylase binding
GO:0044255 cellular lipid metabolic process
GO:0045786 negative regulation of cell cycle
GO:0046329 negative regulation of JNK cascade
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051225 spindle assembly
Regulatory
For research purposes only

Applications of Histone Deacetylase 3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation 1/20 1/100
Immunofluorescence 1/50 1/200
Immunohistology - Paraffin 1/50 1/100
Immunoprecipitation 1/50 1/200
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti histone deacetylase 3 antibody detects a band of approximately 49 kDa in cell lysates under reducing conditions

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*