Rabbit anti Histone deacetylase 2 (pSer394) antibody
recognizes histone deacetylase 2, also known as HDAC2, when phosphorylated at serine 394. Histone deacetylase 2 is a class 1 histone deacetylase. Histone deacetylases are chromatin-modifying enzymes that are involved in regulation of many aspects of cell biology including tissue differentiation, autophagy, apoptosis, migration, mitosis and angiogenesis.
Phosphorylation at serine 394 is involved in the regulation of the enzymatic activity of histone deacetylase 2 (Tsai and Seto 2002
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to histone deacetylase 2 (pSer394) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 394 of human histone deacetylase 2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
histone deacetylase activity
maintenance of chromatin silencing
transcription factor binding
positive regulation of cell proliferation
positive regulation of gene-specific transcription from RNA polymerase II promoter
negative regulation of gene-specific transcription from RNA polymerase II promoter
positive regulation of receptor biosynthetic process
transcription activator activity
specific transcriptional repressor activity
positive regulation of collagen biosynthetic process
negative regulation of transcription factor activity
sequence-specific DNA binding
negative regulation of MHC class II biosynthetic process
negative regulation of cell cycle
positive regulation of proteolysis
nerve growth factor receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Histone Deacetylase 2 (pSer394) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Tsai, S.C. & Seto, E. (2002) Regulation of histone deacetylase 2 by protein kinase CK2.
J Biol Chem. 277 (35): 31826-33.