Histone Deacetylase 2 (pSer394) antibody

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Rabbit anti Histone Deacetylase 2 (pSer394)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2631 P WB 50 µl
Rabbit anti Histone deacetylase 2 (pSer394) antibody recognizes histone deacetylase 2, also known as HDAC2, when phosphorylated at serine 394. Histone deacetylase 2 is a class 1 histone deacetylase. Histone deacetylases are chromatin-modifying enzymes that are involved in regulation of many aspects of cell biology including tissue differentiation, autophagy, apoptosis, migration, mitosis and angiogenesis.

Phosphorylation at serine 394 is involved in the regulation of the enzymatic activity of histone deacetylase 2 (Tsai and Seto 2002).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to histone deacetylase 2 (pSer394) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 394 of human histone deacetylase 2

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q92769 Related reagents
Entrez Gene
HDAC2 Related reagents
GO Terms
GO:0003682 chromatin binding
GO:0007596 blood coagulation
GO:0004407 histone deacetylase activity
GO:0005737 cytoplasm
GO:0006338 chromatin remodeling
GO:0006344 maintenance of chromatin silencing
GO:0008134 transcription factor binding
GO:0008284 positive regulation of cell proliferation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010870 positive regulation of receptor biosynthetic process
GO:0016358 dendrite development
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0016575 histone deacetylation
GO:0016580 Sin3 complex
GO:0016581 NuRD complex
GO:0019899 enzyme binding
GO:0032967 positive regulation of collagen biosynthetic process
GO:0035098 ESC/E(Z) complex
GO:0043433 negative regulation of transcription factor activity
GO:0043565 sequence-specific DNA binding
GO:0045347 negative regulation of MHC class II biosynthetic process
GO:0045786 negative regulation of cell cycle
GO:0045862 positive regulation of proteolysis
GO:0048011 nerve growth factor receptor signaling pathway
Regulatory
For research purposes only

Applications of Histone Deacetylase 2 (pSer394) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Tsai, S.C. & Seto, E. (2002) Regulation of histone deacetylase 2 by protein kinase CK2.
    J Biol Chem. 277 (35): 31826-33.