Histone Deacetylase 1 antibody
Rabbit anti Histone Deacetylase 1
Histone deacetylase 1 antibody recognizes histone deacetylase 1, also known as transcriptional regulator homolog RPD3. The antibody has been extensively validated for western blotting using whole cell lysates.
Histone Deacetylase 1 Antibody with Control Lysate
Histone deacetylase 1 antibody recognizes histone deacetylase 1, also known as transcriptional regulator homolog RPD3. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00175 | WB | 100 µl | ||||
| VPA00175KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human histone deacetylase 1 antibody recognizes histone deacetylase 1, also known as HDAC1.
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA.
Rabbit anti Human histone deacetylase 1 antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA.
Rabbit anti Human histone deacetylase 1 antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti histone deacetylase 1 detects a band of approximately 62 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol - Immunogen
- Synthetic peptide corresponding to an internal region of human histone deacetylase 1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q13547 Related reagents
- Entrez Gene
- HDAC1 Related reagents
- GO Terms
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0007596 blood coagulation
- GO:0004407 histone deacetylase activity
- GO:0005829 cytosol
- GO:0006338 chromatin remodeling
- GO:0006916 anti-apoptosis
- GO:0008134 transcription factor binding
- GO:0008284 positive regulation of cell proliferation
- GO:0042802 identical protein binding
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010870 positive regulation of receptor biosynthetic process
- GO:0016563 transcription activator activity
- GO:0016566 specific transcriptional repressor activity
- GO:0016580 Sin3 complex
- GO:0016581 NuRD complex
- GO:0042826 histone deacetylase binding
- GO:0043922 negative regulation by host of viral transcription
- GO:0045786 negative regulation of cell cycle
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0070932 histone H3 deacetylation
- GO:0070933 histone H4 deacetylation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Histone Deacetylase 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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