Histone Acetyltransferase 1 antibody
Rabbit anti Histone Acetyltransferase 1
Histone acetyltransferase 1 antibody recognizes histone acetyltransferase type B catalytic subunit, also known as KAT1 or HAT1. This antibody has been extensively validated for western blotting using whole cell lysates.
Histone Acetyltransferase 1 Antibody with Control Lysate
Histone acetyltransferase 1 antibody recognizes histone acetyltransferase type B catalytic subunit, also known as KAT1 or HAT1. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00244 | WB | 100 µl | ||||
| VPA00244KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human histone acetyltransferase 1 antibody recognizes histone acetyltransferase 1, also known as histone acetyltransferase type B catalytic subunit.
Encoded by the HAT1 gene, histone acetyltransferase 1 is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for HAT1 (provided by RefSeq, Jun 2009).
Rabbit anti Human histone acetyltransferase 1 antibody detects a band of 49 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the HAT1 gene, histone acetyltransferase 1 is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for HAT1 (provided by RefSeq, Jun 2009).
Rabbit anti Human histone acetyltransferase 1 antibody detects a band of 49 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Preparation
- 20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide between 389-419 amino acids from the C-terminal region of human histone acetyltransferase 1
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- O14929 Related reagents
- Entrez Gene
- HAT1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0004402 histone acetyltransferase activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006323 DNA packaging
- GO:0006348 chromatin silencing at telomere
- GO:0016573 histone acetylation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Histone Acetyltransferase 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Anti histone acetyltransferase 1 detects a band of approximately 49 kDa in Jurkat cell lysates
- Western Blotting
- Anti histone acetyltransferase 1 detects a band of approximately 49 kDa in Jurkat cell lysates
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
