High Mobility Group Protein B2 antibody

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Rabbit anti High Mobility Group Protein B2

Rabbit anti-human high mobility group protein B2 antibody recognizes high mobility group protein B2, also known as HMG-2 or high-mobility group box 2. This antibody has been extensively validated for western blotting using whole cell lysates.

High Mobility Group Protein B2 Antibody with Control Lysate

Rabbit anti-human high mobility group protein B2 antibody recognizes high mobility group protein B2, also known as HMG-2 or high-mobility group box 2. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00494 WB 100 µl
VPA00494KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human high mobility group protein B2 antibody recognizes high mobility group protein B2, also known as HMG-2, high mobility group protein 2, high-mobility group (nonhistone chromosomal) protein 2 or high-mobility group box 2.

HMGB2 gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles (Paull et al. 1993). These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination (Nagaki et al. 1998) (provided by RefSeq, Jul 2008).

Rabbit anti Human high mobility group protein B2 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti high mobility group protein B2 detects a band of approximately 28 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the C terminal region of human high mobility group protein B2
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P26583 Related reagents
Entrez Gene
HMGB2 Related reagents
GO Terms
GO:0000793 condensed chromosome
GO:0001938 positive regulation of endothelial cell proliferation
GO:0003684 damaged DNA binding
GO:0003690 double-stranded DNA binding
GO:0003697 single-stranded DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006334 nucleosome assembly
GO:0005615 extracellular space
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006265 DNA topological change
GO:0006288 base-excision repair, DNA ligation
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0008301 DNA bending activity
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016565 general transcriptional repressor activity
GO:0048015 phosphatidylinositol-mediated signaling
GO:0032075 positive regulation of nuclease activity
GO:0033151 V(D)J recombination
GO:0042056 chemoattractant activity
GO:0043234 protein complex
GO:0043388 positive regulation of DNA binding
GO:0045648 positive regulation of erythrocyte differentiation
GO:0045654 positive regulation of megakaryocyte differentiation
GO:0048471 perinuclear region of cytoplasm
GO:0050786 RAGE receptor binding
GO:0060326 cell chemotaxis
GO:0071222 cellular response to lipopolysaccharide
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of High Mobility Group Protein B2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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