High Mobility Group Protein B2 antibody

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Anti High Mobility Group Protein B2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti High Mobility Group Protein B2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti High Mobility Group Protein B2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of High Mobility Group Protein B2 knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Rabbit anti High Mobility Group Protein B2 antibody (**VPA00494**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 29 second and (B) 30 second exposure.

Anti High Mobility Group Protein B2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Normalized signal intensity of High Mobility Group Protein B2 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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Rabbit anti High Mobility Group Protein B2

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: High Mobility Group Protein B2

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Product Code Applications Pack Size List Price Your Price Qty

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VPA00494 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human high mobility group protein B2 antibody recognizes high mobility group protein B2, also known as HMG-2, high mobility group protein 2, high-mobility group (non-histone chromosomal) protein 2 or high-mobility group box 2.

HMG-2 gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro HMG-2 is able to efficiently bend DNA and form DNA circles (Paull et al. 1993) suggesting a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. HMG-B2 is involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination (Nagaki et al. 1998) .

Rabbit anti Human high mobility group protein B2 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti high mobility group protein B2 detects a band of approximately 28 kDa in HEK293 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose.
Immunogen: Synthetic peptide directed towards the C terminal region of human high mobility group protein B2
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P26583
Entrez Gene: HMGB2
GO Terms: GO:0000793 condensed chromosome; GO:0001938 positive regulation of endothelial cell proliferation; GO:0003684 damaged DNA binding; GO:0003690 double-stranded DNA binding; GO:0003697 single-stranded DNA binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0006334 nucleosome assembly; GO:0005615 extracellular space; GO:0005654 nucleoplasm; View More GO Terms; GO:0005730 nucleolus; GO:0006265 DNA topological change; GO:0006288 base-excision repair, DNA ligation; GO:0006309 DNA fragmentation involved in apoptotic nuclear change; GO:0008301 DNA bending activity; GO:0010843 promoter binding; GO:0016563 transcription activator activity; GO:0016565 general transcriptional repressor activity; GO:0048015 phosphatidylinositol-mediated signaling; GO:0032075 positive regulation of nuclease activity; GO:0033151 V(D)J recombination; GO:0042056 chemoattractant activity; GO:0043234 protein complex; GO:0043388 positive regulation of DNA binding; GO:0045648 positive regulation of erythrocyte differentiation; GO:0045654 positive regulation of megakaryocyte differentiation; GO:0048471 perinuclear region of cytoplasm; GO:0050786 RAGE receptor binding; GO:0060326 cell chemotaxis; GO:0071222 cellular response to lipopolysaccharide