HEXIM1 antibody
Goat anti HEXIM1
HEXIM1 antibody recognizes protein HEXIM1, also known as cardiac lineage protein 1, estrogen down-regulated gene 1 protein, hexamethylene bis-acetamide-inducible protein 1 or menage a quatre protein 1. The antibody has been extensively validated for western blotting using whole cell lysates.
HEXIM1 Antibody with Control Lysate
HEXIM1 antibody recognizes protein HEXIM1, also known as cardiac lineage protein 1, estrogen down-regulated gene 1 protein, hexamethylene bis-acetamide-inducible protein 1 or menage a quatre protein 1. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00125 | WB | 100 µl | ||||
| VPA00125KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human HEXIM1 antibody recognizes protein HEXIM1, also known as cardiac lineage protein 1, estrogen down-regulated gene 1 protein, hexamethylene bis-acetamide-inducible protein 1 or menage a quatre protein 1.
Expression of HEXIM1 is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. HEXIM1 has no introns (provided by RefSeq, Jul 2008).
Goat anti Human HEXIM1 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Expression of HEXIM1 is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. HEXIM1 has no introns (provided by RefSeq, Jul 2008).
Goat anti Human HEXIM1 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti HEXIM1 detects a band of approximately 70 kDa in K562 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography.
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5 % BSA
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5 % BSA
- Immunogen
- Peptide with the sequence C-HRQQERAPLSKFGD, from the C Terminus of the protein sequence.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- O94992 Related reagents
- Entrez Gene
- HEXIM1 Related reagents
- GO Terms
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0005515 protein binding
- GO:0004861 cyclin-dependent protein kinase inhibitor activity
- GO:0017069 snRNA binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006350 transcription
- GO:0045736 negative regulation of cyclin-dependent protein kinase activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of HEXIM1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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