HEREMANS-SCHMID Glycoprotein antibody

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Goat anti HEREMANS-SCHMID Glycoprotein

Goat anti human Heremans-Schmid glycoprotein antibody recognizes alpha-2 Heremans-Schmid glycoprotein, also known as fetuin A. This antibody has been extensively validated for western blotting using whole cell lysates.

HEREMANS-SCHMID Glycoprotein Antibody with Control Lysate

Goat anti human Heremans-Schmid glycoprotein antibody recognizes alpha-2 Heremans-Schmid glycoprotein, also known as fetuin A. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00153 WB 100 µl
VPA00153KT WB 2 Westerns
With Lysate 400µg
Goat anti Human Heremans-Schmid glycoprotein antibody recognizes Heremans-Schmid glycoprotein also known as Alpha-2-HS-glycoprotein , Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A and it is cleaved into the following 2 chains: Alpha-2-HS-glycoprotein chain A and Alpha-2-HS-glycoprotein chain B.

Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure (provided by RefSeq, Jul 2008).

Goat anti Human Heremans-Schmid glycoprotein antibody detects a band of 58 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti Heremans-Schmid glycoprotein detects a band of approximately 58 kDa in C6 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody prepared by ion exchange chromatography
Preparation
20μl Goat polyclonal antibody prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% BSA
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Recombinant human Heremans-Schmid glycoprotein processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg C6 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P02765 Related reagents
Entrez Gene
AHSG Related reagents
GO Terms
GO:0005515 protein binding
GO:0001501 skeletal system development
GO:0006953 acute-phase response
GO:0004869 cysteine-type endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0006907 pinocytosis
GO:0019210 kinase inhibitor activity
GO:0030502 negative regulation of bone mineralization
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0050727 regulation of inflammatory response
GO:0050766 positive regulation of phagocytosis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of HEREMANS-SCHMID Glycoprotein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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