Hemoglobin Subunit Epsilon antibody

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Rabbit anti Hemoglobin Subunit Epsilon

Rabbit anti-human hemoglobin subunit epsilon antibody recognizes hemoglobin subunit epsilon, also known as epsilon globin. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00539 WB 100 µl
Rabbit anti Human hemoglobin subunit epsilon antibody recognizes hemoglobin subunit epsilon also known as epsilon globin.

The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3' (provided by RefSeq, Jul 2008).

Rabbit anti Human hemoglobin subunit epsilon antibody detects a band of 14 kDa. The antibody has been extensively validated for western blotting using tissue and whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti hemoglobin subunit epsilon detects a band of approximately 14 kDa in mouse liver
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 55-83 of human hemoglobin subunit epsilon
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P02100 Related reagents
Entrez Gene
HBE1 Related reagents
GO Terms
GO:0005344 oxygen transporter activity
GO:0007596 blood coagulation
GO:0005833 hemoglobin complex
GO:0019825 oxygen binding
GO:0020037 heme binding
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Hemoglobin Subunit Epsilon antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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