Hemoglobin antibody

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Sheep anti Human Hemoglobin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4870-3979G E WB 1 ml
Sheep anti Human Hemoglobin antibody recognizes human hemoglobin, a ~66 kDa metalloprotein composed of four globular subunits each of which is associated with an iron-containing heme group. The heme group is involved with oxygen transport from the lungs to the tissues and the globin molecule plays a major role in transporting carbon dioxide in the reverse direction.

Hemoglobin is found in the erythrocytes of all vertebrates. There is a wide diversity of amino acid sequences and substitutions within mammalian hemoglobins, however the molecular weight is generally around 66 kDa with an iron content of about 0.34%. The level of hemoglobin in the blood is used in the diagnosis of anemia; levels in the feces are indicative of various clinical conditions.

Sheep anti Human Hemoglobin antibody shows <10% cross-reactivity with bovine hemoglobin.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Weak
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human hemoglobin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography on Protein G.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Hemoglobin purified from human erythrocytes
Approx. Protein Concentrations
IgG concentration 5.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P68871 Related reagents
P69905 Related reagents
Entrez Gene
HBB Related reagents
HBA2 Related reagents
GO Terms
GO:0022627 cytosolic small ribosomal subunit
GO:0005515 protein binding
GO:0005344 oxygen transporter activity
GO:0005833 hemoglobin complex
GO:0010942 positive regulation of cell death
GO:0019825 oxygen binding
GO:0020037 heme binding
GO:0042744 hydrogen peroxide catabolic process
GO:0051291 protein heterooligomerization
Regulatory
For research purposes only

Applications of Hemoglobin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB