Hemoglobin antibody

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Anti Human Hemoglobin Antibody gallery image 1 — Human hemoglobin detected in a red blood cell lysate using Sheep anti Human Hemoglobin (**4870-3979G**). (Other bands detected represent different subunits of hemoglobin).

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Sheep anti Human Hemoglobin

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Hemoglobin

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4870-3979G Datasheet Datasheet SDS SDS	E WB	1 ml		Log in	Get Quote
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Sheep anti Human Hemoglobin antibody recognizes human hemoglobin, a ~66 kDa metalloprotein composed of four globular subunits each of which is associated with an iron-containing heme group. The heme group is involved with oxygen transport from the lungs to the tissues and the globin molecule plays a major role in transporting carbon dioxide in the reverse direction.

Hemoglobin is found in the erythrocytes of all vertebrates. There is a wide diversity of amino acid sequences and substitutions within mammalian hemoglobins, however the molecular weight is generally around 66 kDa with an iron content of about 0.34%. The level of hemoglobin in the blood is used in the diagnosis of anemia; levels in the feces are indicative of various clinical conditions.

Sheep anti Human Hemoglobin antibody shows <10% cross-reactivity with bovine hemoglobin.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Bovine Weak; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antisera to human hemoglobin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography on Protein G.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Hemoglobin purified from human erythrocytes
Approx. Protein Concentrations: IgG concentration 5.0mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Bovine	Weak

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Western Blotting		1/100	1/1000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Description	Product Code	Applications	Pack Size	Your Price
Rabbit anti Sheep IgG (H/L):Biotin	5184-2304	C E F WB	1.5 ml	Log in	Get Quote
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Description	Rabbit anti Sheep IgG (H/L):Biotin

RRID: AB_2114441
UniProt: P68871; P69905
Entrez Gene: HBB; HBA2
GO Terms: GO:0022627 cytosolic small ribosomal subunit; GO:0005515 protein binding; GO:0005344 oxygen transporter activity; GO:0005833 hemoglobin complex; GO:0010942 positive regulation of cell death; GO:0019825 oxygen binding; GO:0020037 heme binding; GO:0042744 hydrogen peroxide catabolic process; GO:0051291 protein heterooligomerization; View More GO Terms