Hemoglobin antibody
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WB ResetSheep anti Human Hemoglobin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Hemoglobin
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| Sheep anti Human Hemoglobin antibody recognizes human hemoglobin, a ~66 kDa metalloprotein composed of four globular subunits each of which is associated with an iron-containing heme group. The heme group is involved with oxygen transport from the lungs to the tissues and the globin molecule plays a major role in transporting carbon dioxide in the reverse direction. Hemoglobin is found in the erythrocytes of all vertebrates. There is a wide diversity of amino acid sequences and substitutions within mammalian hemoglobins, however the molecular weight is generally around 66 kDa with an iron content of about 0.34%. The level of hemoglobin in the blood is used in the diagnosis of anemia; levels in the feces are indicative of various clinical conditions. Sheep anti Human Hemoglobin antibody shows <10% cross-reactivity with bovine hemoglobin. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Weak - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human hemoglobin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography on Protein G.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Hemoglobin purified from human erythrocytes
- Approx. Protein Concentrations
- IgG concentration 5.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
1/100 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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- RRID
- AB_2114441
- UniProt
- P68871
- P69905
- Entrez Gene
- HBB
- HBA2
- GO Terms
- GO:0022627 cytosolic small ribosomal subunit
- GO:0005515 protein binding
- GO:0005344 oxygen transporter activity
- GO:0005833 hemoglobin complex
- GO:0010942 positive regulation of cell death
- GO:0019825 oxygen binding
- GO:0020037 heme binding
- GO:0042744 hydrogen peroxide catabolic process
- GO:0051291 protein heterooligomerization
- View More GO Terms
View more products with Hemoglobin specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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