Hemoglobin antibody
Sheep anti Human Hemoglobin antibody recognizes human hemoglobin, a ~66 kDa metalloprotein composed of four globular subunits each of which is associated with an iron-containing heme group. The heme group is involved with oxygen transport from the lungs to the tissues and the globin molecule plays a major role in transporting carbon dioxide in the reverse direction.
Hemoglobin is found in the erythrocytes of all vertebrates. There is a wide diversity of amino acid sequences and substitutions within mammalian hemoglobins, however the molecular weight is generally around 66 kDa with an iron content of about 0.34%. The level of hemoglobin in the blood is used in the diagnosis of anemia; levels in the feces are indicative of various clinical conditions.
Sheep anti Human Hemoglobin antibody shows <10% cross-reactivity with bovine hemoglobin.
Hemoglobin is found in the erythrocytes of all vertebrates. There is a wide diversity of amino acid sequences and substitutions within mammalian hemoglobins, however the molecular weight is generally around 66 kDa with an iron content of about 0.34%. The level of hemoglobin in the blood is used in the diagnosis of anemia; levels in the feces are indicative of various clinical conditions.
Sheep anti Human Hemoglobin antibody shows <10% cross-reactivity with bovine hemoglobin.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Weak - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human hemoglobin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography on Protein G.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Hemoglobin purified from human erythrocytes
- Approx. Protein Concentrations
- IgG concentration 5.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P68871
- P69905
- Entrez Gene
- HBB
- HBA2
- GO Terms
- GO:0022627 cytosolic small ribosomal subunit
- GO:0005515 protein binding
- GO:0005344 oxygen transporter activity
- GO:0005833 hemoglobin complex
- GO:0010942 positive regulation of cell death
- GO:0019825 oxygen binding
- GO:0020037 heme binding
- GO:0042744 hydrogen peroxide catabolic process
- GO:0051291 protein heterooligomerization
- Regulatory
- For research purposes only
Applications of Hemoglobin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting | 1/100 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
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| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
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