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Heme Oxygenase 1 antibody

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Western Blot Validated
Anti Heme Oxygenase 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with heme oxygenase 1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 4 second exposure. Arrow points to heme oxygenase 1 (molecular weight 28 kDa).

Rabbit anti Heme Oxygenase 1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00553
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human heme oxygenase 1 antibody recognizes heme oxygenase 1, also known as heat shock protein, 32-kD or HO1.

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family (provided by RefSeq, Jul 2008).

Rabbit anti Human heme oxygenase 1 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti heme oxygenase 1 detects a band of approximately 28 kDa in A549 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Human heme oxygenase 1 synthetic multiple antigenic peptide

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P09601
Entrez Gene
HMOX1
GO Terms
GO:0001525 angiogenesis
GO:0001935 endothelial cell proliferation
GO:0002246 wound healing involved in inflammatory response
GO:0002686 negative regulation of leukocyte migration
GO:0004392 heme oxygenase (decyclizing) activity
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0006788 heme oxidation
GO:0006879 cellular iron ion homeostasis
GO:0006916 anti-apoptosis
GO:0007243 intracellular protein kinase cascade
GO:0007588 excretion
GO:0008219 cell death
GO:0045909 positive regulation of vasodilation
GO:0014806 smooth muscle hyperplasia
GO:0019899 enzyme binding
GO:0020037 heme binding
GO:0034101 erythrocyte homeostasis
GO:0034383 low-density lipoprotein particle clearance
GO:0035094 response to nicotine
GO:0042167 heme catabolic process
GO:0042542 response to hydrogen peroxide
GO:0042803 protein homodimerization activity
GO:0051260 protein homooligomerization
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045765 regulation of angiogenesis
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0055085 transmembrane transport
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of Heme Oxygenase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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