Heme Oxygenase 1 antibody

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Rabbit anti Heme Oxygenase 1

<strong>Rabbit anti-human heme oxygenase 1 antibody</strong> recognizes heme oxygenase 1, also known as heat shock protein, 32-kDa. This antibody has been extensively validated for western blotting using whole cell lysates.

Heme Oxygenase 1 Antibody with Control Lysate

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size
VPA00553	WB	100 µl
VPA00553KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Rabbit anti Human heme oxygenase 1 antibody recognizes heme oxygenase 1, also known as heat shock protein, 32-kD or HO1.

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family (provided by RefSeq, Jul 2008).

Rabbit anti Human heme oxygenase 1 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Rabbit polyclonal antibody purified by affinity chromatography

Preparation

20μl Rabbit polyclonal antibody purified by affinity chromatography

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Human heme oxygenase 1 synthetic multiple antigenic peptide

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch
Shelf Life: As supplied, 12 months from date of despatch

More Information

UniProt: P09601 Related reagents
Entrez Gene: HMOX1 Related reagents
GO Terms: GO:0001525 angiogenesis; GO:0001935 endothelial cell proliferation; GO:0002246 wound healing involved in inflammatory response; GO:0002686 negative regulation of leukocyte migration; GO:0004392 heme oxygenase (decyclizing) activity; GO:0004871 signal transducer activity; GO:0005615 extracellular space; GO:0005789 endoplasmic reticulum membrane; GO:0005792 microsome; GO:0006788 heme oxidation; GO:0006879 cellular iron ion homeostasis; GO:0006916 anti-apoptosis; GO:0007243 intracellular protein kinase cascade; GO:0007588 excretion; GO:0008219 cell death; GO:0045909 positive regulation of vasodilation; GO:0014806 smooth muscle hyperplasia; GO:0019899 enzyme binding; GO:0020037 heme binding; GO:0034101 erythrocyte homeostasis; GO:0034383 low-density lipoprotein particle clearance; GO:0035094 response to nicotine; GO:0042167 heme catabolic process; GO:0042542 response to hydrogen peroxide; GO:0042803 protein homodimerization activity; GO:0051260 protein homooligomerization; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress; GO:0045080 positive regulation of chemokine biosynthetic process; GO:0045765 regulation of angiogenesis; GO:0048661 positive regulation of smooth muscle cell proliferation; GO:0048662 negative regulation of smooth muscle cell proliferation; GO:0055085 transmembrane transport
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of Heme Oxygenase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Anti heme oxygenase 1 detects a band of approximately 28 kDa in A549 cell lysates
Western Blotting: Anti heme oxygenase 1 detects a band of approximately 28 kDa in A549 cell lysates
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB

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