Heme Oxygenase 1 antibody

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Rabbit anti Heme Oxygenase 1

<strong>Rabbit anti-human heme oxygenase 1 antibody</strong> recognizes heme oxygenase 1, also known as heat shock protein, 32-kDa. This antibody has been extensively validated for western blotting using whole cell lysates.

Heme Oxygenase 1 Antibody with Control Lysate

<strong>Rabbit anti-human heme oxygenase 1 antibody</strong> recognizes heme oxygenase 1, also known as heat shock protein, 32-kDa. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00553 WB 100 µl
VPA00553KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human heme oxygenase 1 antibody recognizes heme oxygenase 1, also known as heat shock protein, 32-kD or HO1.

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family (provided by RefSeq, Jul 2008).

Rabbit anti Human heme oxygenase 1 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human heme oxygenase 1 synthetic multiple antigenic peptide

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P09601 Related reagents
Entrez Gene
HMOX1 Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0001935 endothelial cell proliferation
GO:0002246 wound healing involved in inflammatory response
GO:0002686 negative regulation of leukocyte migration
GO:0004392 heme oxygenase (decyclizing) activity
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0006788 heme oxidation
GO:0006879 cellular iron ion homeostasis
GO:0006916 anti-apoptosis
GO:0007243 intracellular protein kinase cascade
GO:0007588 excretion
GO:0008219 cell death
GO:0045909 positive regulation of vasodilation
GO:0014806 smooth muscle hyperplasia
GO:0019899 enzyme binding
GO:0020037 heme binding
GO:0034101 erythrocyte homeostasis
GO:0034383 low-density lipoprotein particle clearance
GO:0035094 response to nicotine
GO:0042167 heme catabolic process
GO:0042542 response to hydrogen peroxide
GO:0042803 protein homodimerization activity
GO:0051260 protein homooligomerization
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045765 regulation of angiogenesis
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0055085 transmembrane transport
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Heme Oxygenase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti heme oxygenase 1 detects a band of approximately 28 kDa in A549 cell lysates
Western Blotting
Anti heme oxygenase 1 detects a band of approximately 28 kDa in A549 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB