Heme Oxygenase 1 antibody

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Anti Heme Oxygenase 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Rabbit anti Human heme oxygenase 1 antibody (**VPA00553**) followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to heme oxygenase 1 (molecular weight 28 kDa).

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Rabbit anti Heme Oxygenase 1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Heme Oxygenase 1

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VPA00553 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human heme oxygenase 1 antibody recognizes heme oxygenase 1, also known as heat shock protein, 32-kD or HO1.

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family (provided by RefSeq, Jul 2008).

Rabbit anti Human heme oxygenase 1 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti heme oxygenase 1 detects a band of approximately 28 kDa in A549 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
25% Glycerol
Immunogen: Human heme oxygenase 1 synthetic multiple antigenic peptide
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P09601
Entrez Gene: HMOX1
GO Terms: GO:0001525 angiogenesis; GO:0001935 endothelial cell proliferation; GO:0002246 wound healing involved in inflammatory response; GO:0002686 negative regulation of leukocyte migration; GO:0004392 heme oxygenase (decyclizing) activity; GO:0004871 signal transducer activity; GO:0005615 extracellular space; GO:0005789 endoplasmic reticulum membrane; GO:0005792 microsome; View More GO Terms; GO:0006788 heme oxidation; GO:0006879 cellular iron ion homeostasis; GO:0006916 anti-apoptosis; GO:0007243 intracellular protein kinase cascade; GO:0007588 excretion; GO:0008219 cell death; GO:0045909 positive regulation of vasodilation; GO:0014806 smooth muscle hyperplasia; GO:0019899 enzyme binding; GO:0020037 heme binding; GO:0034101 erythrocyte homeostasis; GO:0034383 low-density lipoprotein particle clearance; GO:0035094 response to nicotine; GO:0042167 heme catabolic process; GO:0042542 response to hydrogen peroxide; GO:0042803 protein homodimerization activity; GO:0051260 protein homooligomerization; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress; GO:0045080 positive regulation of chemokine biosynthetic process; GO:0045765 regulation of angiogenesis; GO:0048661 positive regulation of smooth muscle cell proliferation; GO:0048662 negative regulation of smooth muscle cell proliferation; GO:0055085 transmembrane transport