HEC1 antibody

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Rabbit anti HEC1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00689
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human HEC1 antibody recognizes the kinetochore protein NDC80 homolog, also known as kinetochore complex component, HEC1, kinetochore associated 2, kinetochore protein Hec1 or retinoblastoma-associated protein HEC.

The NDC80 gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. (provided by RefSeq, Oct 2011).

Rabbit anti Human HEC1 antibody detects a band of 76 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti HEC1 detects a band of approximately 76 kDa in Jurkat cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
KLH conjugated synthetic peptide encompassing part of human HEC1 (amino acids 594 - 628)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
O14777
Entrez Gene
NDC80
GO Terms
GO:0000070 mitotic sister chromatid segregation
GO:0000132 establishment of mitotic spindle orientation
GO:0005515 protein binding
GO:0000236 mitotic prometaphase
GO:0000942 condensed nuclear chromosome outer kinetochore
GO:0005829 cytosol
GO:0007052 mitotic spindle organization
GO:0008608 attachment of spindle microtubules to kinetochore
GO:0048015 phosphatidylinositol-mediated signaling
GO:0031262 Ndc80 complex
GO:0051301 cell division
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of HEC1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml