Heat Shock Protein 90 Alpha antibody

Rabbit anti Human Heat Shock Protein 90 Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Heat Shock Protein 90 Alpha

Product Code Applications Pack Size List Price Your Price Qty
AHP1339
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SDS Safety Datasheet SDS
E WB 50 µg loader
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Rabbit anti Human heat shock protein 90 alpha antibody detects human heat shock protein 90 alpha (HSP90α), one of two HSP90 isoforms together with HSP90β. These isoforms are encoded by different genes and under normal conditions assist the folding of newly synthesized proteins, and are also important in cellular processes such as hormone signaling and cell cycle control.

In response to mutation or environmental stress, HSP90 expression is upregulated as part of the heat shock response, and function to maintain newly destabilized proteins in a folding competent state. HSP90 can also affect the epigenetic state at specific loci, leading to phenotype modulation.

Induction of HSP90α by c-Myc may control the activity of multiple signal pathways involved in cellular transformation.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Monkey Expected from Sequence
Chicken Expected from Sequence
Mouse Expected from Sequence
Drosophila Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human heat shock protein 90 alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 289-300 of human heat shock protein 90 alpha.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/25000
Immunoprecipitation
Western Blotting 1/500 1/2500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1339 detects a band of approximately 90kDa.

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References for Heat Shock Protein 90 Alpha antibody

  1. Teng, S.C. et al. (2004) Direct activation of HSP90A transcription by c-Myc contributes to c-Myc-induced transformation.
    J Biol Chem. 279 (15): 14649-55.
  2. Chen, W.S. et al. (2013) Secreted heat shock protein 90α (HSP90α) induces nuclear factor-κB-mediated TCF12 protein expression to down-regulate E-cadherin and to enhance colorectal cancer cell migration and invasion.
    J Biol Chem. 288 (13): 9001-10.
  3. Fan, C.S. et al. (2022) Extracellular HSP90α Induces MyD88-IRAK Complex-Associated IKKα/β-NF-κB/IRF3 and JAK2/TYK2-STAT-3 Signaling in Macrophages for Tumor-Promoting M2-Polarization.
    Cells. 11 (2)Jan 11 [Epub ahead of print].

Synonyms
HSP90A
RRID
AB_906044
UniProt
P07900
Entrez Gene
HSP90AA1
GO Terms
GO:0051082 unfolded protein binding
GO:0005886 plasma membrane
GO:0000086 G2/M transition of mitotic cell cycle
GO:0006986 response to unfolded protein
GO:0042026 protein refolding
GO:0005829 cytosol
GO:0007165 signal transduction
GO:0005524 ATP binding
GO:0007411 axon guidance
GO:0030235 nitric-oxide synthase regulator activity
GO:0030911 TPR domain binding
GO:0034619 cellular chaperone-mediated protein complex assembly
GO:0042470 melanosome
GO:0042803 protein homodimerization activity
GO:0045040 protein import into mitochondrial outer membrane
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0046209 nitric oxide metabolic process
GO:0050999 regulation of nitric-oxide synthase activity