Heat Shock Protein 90 Alpha antibody
Rabbit anti Human heat shock protein 90 alpha antibody (AHP1339) used to demonstrate the physical association of HSP90α with TLR4 and CD91 on macrophages in culture with a proximity ligation assay.
Image caption:
The canonical CD91/MyD88–IRAK1/4–IKKα/β–NF-κB/IRF3 signaling pathway is involved in eHSP90α-induced macrophage M2-polarization. (A) Red fluorescent dots in PLAs showed that the physical associations of HSP90α–TLR4, HSP90α–CD91, TLR4–MyD88, CD91–MyD88, CD91–IRAK1, and MyD88–IRAK1 were induced when THP-1-derived macrophages were treated for 4 h with 15 μg/mL of rHSP90α. The representative data of three independent experiments are shown.
From: Fan CS, Chen CC, Chen LL, Chua KV, Hung HC, Hsu JT, Huang TS. Extracellular HSP90α Induces MyD88-IRAK Complex-Associated IKKα/β-NF-κB/IRF3 and JAK2/TYK2-STAT-3 Signaling in Macrophages for Tumor-Promoting M2-Polarization.
Cells. 2022 Jan 11;11(2):229.
doi: 10.3390/cells11020229.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
PLA IF ResetRabbit anti Human Heat Shock Protein 90 Alpha
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Heat Shock Protein 90 Alpha
Antibody Quality and Performance Guarantee
| Rabbit anti Human heat shock protein 90 alpha antibody detects human heat shock protein 90 alpha (HSP90α), one of two HSP90 isoforms together with HSP90β. These isoforms are encoded by different genes and under normal conditions assist the folding of newly synthesized proteins, and are also important in cellular processes such as hormone signaling and cell cycle control. In response to mutation or environmental stress, HSP90 expression is upregulated as part of the heat shock response, and function to maintain newly destabilized proteins in a folding competent state. HSP90 can also affect the epigenetic state at specific loci, leading to phenotype modulation. Induction of HSP90α by c-Myc may control the activity of multiple signal pathways involved in cellular transformation. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Monkey Expected from Sequence Chicken Expected from Sequence Mouse Expected from Sequence Drosophila Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human heat shock protein 90 alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to amino acids 289-300 of human heat shock protein 90 alpha.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/5000 | 1/25000 |
| Immunoprecipitation | |||
| Western Blotting | |
1/500 | 1/2500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1339 detects a band of approximately 90kDa.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Description | Sheep anti Rabbit IgG:FITC | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for Heat Shock Protein 90 Alpha antibody
-
Teng, S.C. et al. (2004) Direct activation of HSP90A transcription by c-Myc contributes to c-Myc-induced transformation.
J Biol Chem. 279 (15): 14649-55. -
Chen, W.S. et al. (2013) Secreted heat shock protein 90α (HSP90α) induces nuclear factor-κB-mediated TCF12 protein expression to down-regulate E-cadherin and to enhance colorectal cancer cell migration and invasion.
J Biol Chem. 288 (13): 9001-10. -
Fan, C.S. et al. (2022) Extracellular HSP90α Induces MyD88-IRAK Complex-Associated IKKα/β-NF-κB/IRF3 and JAK2/TYK2-STAT-3 Signaling in Macrophages for Tumor-Promoting M2-Polarization.
Cells. 11 (2)Jan 11 [Epub ahead of print].
- Synonyms
- HSP90A
- RRID
- AB_906044
- UniProt
- P07900
- Entrez Gene
- HSP90AA1
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0005886 plasma membrane
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0006986 response to unfolded protein
- GO:0042026 protein refolding
- GO:0005829 cytosol
- GO:0007165 signal transduction
- GO:0005524 ATP binding
- GO:0007411 axon guidance
- View More GO Terms
- GO:0030235 nitric-oxide synthase regulator activity
- GO:0030911 TPR domain binding
- GO:0034619 cellular chaperone-mediated protein complex assembly
- GO:0042470 melanosome
- GO:0042803 protein homodimerization activity
- GO:0045040 protein import into mitochondrial outer membrane
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0046209 nitric oxide metabolic process
- GO:0050999 regulation of nitric-oxide synthase activity
AHP1339
170111
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