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Heat Shock 70 Kda Protein 5 antibody

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Anti Heat Shock 70 Kda Protein 5 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1
Anti Heat Shock 70 Kda Protein 5 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Rabbit anti Human heat shock 70 kDa protein 5 antibody (VPA00784) followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 17 second exposure. Arrow points to heat shock 70 kDa protein 5 (molecular weight 78 kDa).

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WB

Rabbit anti Heat Shock 70 Kda Protein 5

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Heat Shock 70 Kda Protein 5

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Product Code Applications Pack Size List Price Your Price Qty
VPA00784
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SDS Safety Datasheet SDS
WB 100 µl loader
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Rabbit anti Human heat shock 70 kDa protein 5 antibody recognizes heat shock 70 kDa protein 5, also known as HSPA5, 78 kDa glucose-regulated protein or immunoglobulin heavy chain-binding protein.

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell (provided by RefSeq, Sep 2010).

Rabbit anti Human heat shock 70 kDa protein 5 detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti heat shock 70 kDa protein 5 antibody recognizes a band of approximately 78 kDa in Raji cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the middle region of human heat shock 70 kDa protein 5
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml
List Price Your Price
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
P11021
Entrez Gene
HSPA5
GO Terms
GO:0051082 unfolded protein binding
GO:0005509 calcium ion binding
GO:0002576 platelet degranulation
GO:0051087 chaperone binding
GO:0030674 protein binding, bridging
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005788 endoplasmic reticulum lumen
GO:0005793 ER-Golgi intermediate compartment
GO:0006916 anti-apoptosis
GO:0009986 cell surface
GO:0030168 platelet activation
GO:0030176 integral to endoplasmic reticulum membrane
GO:0030433 ER-associated protein catabolic process
GO:0030496 midbody
GO:0031625 ubiquitin protein ligase binding
GO:0042149 cellular response to glucose starvation
GO:0042470 melanosome
GO:0043027 caspase inhibitor activity
GO:0043154 negative regulation of caspase activity
GO:0048471 perinuclear region of cytoplasm
GO:0060904 regulation of protein folding in endoplasmic reticulum

VPA00784

170515

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