Haptoglobin antibody

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Sheep anti Human Haptoglobin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP082 ID IF 1 ml
Sheep anti Human haptoglobin antibody recognizes human haptoglobulin, a plasma protein that has high affinity for hemoglobin and is responsible for its capture and hepatic recycling. This process also acts to prevent kidney damage where hemoglobin would otherwise accumulate prior to excretion in the urine. Haptoglobin/hemoglobin complexes are cleared from the circulation in the liver by Kuppfer cells expressing the CD163 scavanger receptor.

Two isoforms of human haptoglobin produced by alternative splicing have been described. Sheep anti Human haptoglobin antibody (AHP082) binds to the canonical isoform 1 of human haptoglobin, binding to the truncated (missing residues 38-96) isoform 2 has not been evaluated.

Mutation of the HP gene can lead to the development of Anhaptoglobinemia (AHP), a disease characterized by an absence of serum haptoglobin (Teye et al. 2004).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Cat
Rabbit
Mouse
Bovine
Dog
Chicken
Horse
Guinea Pig
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human haptoglobulin were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by ion exchange chromatography.
Buffer Solution
Glycine buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.1%EACA
0.01%Benzamidine
1mMEDTA
Immunogen
Human haptoglobulin purified from serum.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P00738 Related reagents
Entrez Gene
HP Related reagents
GO Terms
GO:0006952 defense response
GO:0004252 serine-type endopeptidase activity
GO:0005576 extracellular region
GO:0006508 proteolysis
GO:0006879 cellular iron ion homeostasis
GO:0010942 positive regulation of cell death
GO:0030492 hemoglobin binding
GO:0051354 negative regulation of oxidoreductase activity
GO:2000296 negative regulation of hydrogen peroxide catabolic process
Regulatory
For research purposes only

Applications of Haptoglobin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunodiffusion Neat
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Product Specific References

References for Haptoglobin antibody

  1. Gustafsson, K. et al. (1997) Toxoplasma gondii infection in the mountain hare (Lepus timidus) and domestic rabbit (Oryctolagus cuniculus). II. Early immune reactions.
    J Comp Pathol. 117 (4): 361-9.
  2. Hoagland, L.F. 4th et al. (2007) Haptoglobin and posttranslational glycan-modified derivatives as serum biomarkers for the diagnosis of nonsmall cell lung cancer.
    Cancer. 110 (10): 2260-8.
  3. Merril, C.R. et al. (1995) Diagnosing Alzheimer's disease and schizophrenia
    US patent No 5,429947.
  4. McGuire, W. et al. (1996) C-reactive protein and haptoglobin in the evaluation of a community-based malaria control programme.
    Trans R Soc Trop Med Hyg. 90 (1): 10-4.