GSK3 Alpha (pSer21) antibody
Rabbit anti GSK3 Alpha (pSer21)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- GSK3 Alpha (pSer21)
Antibody Quality and Performance Guarantee
| Rabbit anti GSK3 Alpha (pSer21) antibody recognizes GSK3 alpha when phosphorylated at serine 21. GSK3 is a member of the CMGC family of kinases. GSK3 comprises two isoforms, alpha and beta, with 85% total homology and 98% homology between kinase domains. GSK3 alpha is phosphorylated at serine 21 by protein kinase A, AKT and ribosomal S6 kinases p90RSK and p70S6K. Phosphorylation of GSK3 alpha at serine 21 leads to inactivation of GSK3 alpha. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to GSK3 ALPHA (pSer21) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 21 of human GSK3 ALPHA
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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- UniProt
- P49840
- Entrez Gene
- GSK3A
- GO Terms
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0006468 protein phosphorylation
- GO:0008286 insulin receptor signaling pathway
- GO:0010905 negative regulation of UDP-glucose catabolic process
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030877 beta-catenin destruction complex
- GO:0034236 protein kinase A catalytic subunit binding
- View More GO Terms
- GO:0045732 positive regulation of protein catabolic process
- GO:0046325 negative regulation of glucose import
- GO:0046627 negative regulation of insulin receptor signaling pathway
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050321 tau-protein kinase activity
- GO:0051348 negative regulation of transferase activity
AHP2625
141583
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