GRP94 antibody

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Rabbit anti Human GRP94

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP848 IF WB 0.1 ml
Rabbit anti Human GRP94 antibody recognizes the 94 kDa glucose regulated protein, GRP94, also known as tumour rejection antigen (gp96). GRP94 is an endoplasmic reticulum resident molecular chaperone, a paralog of cytoplasmic Hsp90. GRP94 assists in the folding of membrane-bound proteins including the Toll-like receptors, certain subclasses of integrins and some secreted proteins such as IgG.

Rabbit anti Human GRP94 antibody detects a band of ~105 kDa in cynomolgus monkey brain lysates by western blotting.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Sheep
Cynomolgus monkey
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to human GRP94 were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Synthetic peptide derived from the internal sequence of human GRP94 conjugated to KLH

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P14625 Related reagents
Entrez Gene
HSP90B1 Related reagents
GO Terms
GO:0051082 unfolded protein binding
GO:0005509 calcium ion binding
GO:0001666 response to hypoxia
GO:0003723 RNA binding
GO:0006457 protein folding
GO:0005829 cytosol
GO:0051208 sequestering of calcium ion
GO:0005524 ATP binding
GO:0005788 endoplasmic reticulum lumen
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0006916 anti-apoptosis
GO:0015031 protein transport
GO:0019903 protein phosphatase binding
GO:0030433 ER-associated protein catabolic process
GO:0030496 midbody
GO:0031247 actin rod assembly
GO:0042470 melanosome
GO:0043666 regulation of phosphoprotein phosphatase activity
GO:0046790 virion binding
GO:0048471 perinuclear region of cytoplasm
GO:0050750 low-density lipoprotein particle receptor binding
GO:0071318 cellular response to ATP
Regulatory
For research purposes only

Applications of GRP94 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for GRP94 antibody

  1. Yang, M. et al. (2015) A hyposensitive anticancer drug induces higher surface expression and release of heat shock proteins in a human hepatocellular carcinoma cell line.
    Mol Med Rep. 12 (2): 2879-85.