GRO Gamma antibody
Rabbit anti Human GRO gamma antibody recognizes human GRO-gamma, also known as CXCL3, Growth-regulated protein gamma, GRO-gamma(1-73), Macrophage inflammatory protein 2-beta or MIP2-beta. GRO gamma is a ~7.9 kDa CXC chemokine and one of three closely related, but distinct GRO gene products, which act as chemoattractants and activators of neutrophils and basophils.
Like GRO-alpha (CXCL1) and GRO-beta (CXCL2), GRO-gamma contains the ELR-motif (N-Terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors, though studies have shown a difference in the potency and receptor binding affinity of these three proteins (Wuyts et al. 1999).
Like GRO-alpha (CXCL1) and GRO-beta (CXCL2), GRO-gamma contains the ELR-motif (N-Terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors, though studies have shown a difference in the potency and receptor binding affinity of these three proteins (Wuyts et al. 1999).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to human GRO-gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant human GRO-gamma (PHP117)
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 3 months from date of reconstitution.
Please see label for expiry date.
More Information
- UniProt
- P19876 Related reagents
- Entrez Gene
- CXCL3 Related reagents
- GO Terms
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0008009 chemokine activity
- GO:0030593 neutrophil chemotaxis
- Regulatory
- For research purposes only
Applications of GRO Gamma antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 | 2.0ug/ml | |
| Functional Assays | 1.0 | 2.0ug/ml | |
| Immunohistology - Paraffin 1 | 0.5 | 0.7ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used in an indirect ELISA with PHP117 as the standard.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
References for GRO Gamma antibody
-
Kogan-sakin, I. et al. (2009) Prostate stromal cells produce CXCL-1, CXCL-2, CXCL-3 and IL-8 in response to epithelia-secreted IL-1.
Carcinogenesis. 30 (4): 698-705.
Further Reading
-
Haskill, S. et al. (1990) Identification of three related human GRO genes encoding cytokine functions.
Proc Natl Acad Sci U S A. 87 (19): 7732-6. -
Geiser, T. et al. (1993) The interleukin-8-related chemotactic cytokines GRO alpha, GRO beta, and GRO gamma activate human neutrophil and basophil leukocytes.
J Biol Chem. 268 (21): 15419-24.
