GRO Gamma antibody


Like GRO-alpha (CXCL1) and GRO-beta (CXCL2), GRO-gamma contains the ELR-motif (N-Terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors, though studies have shown a difference in the potency and receptor binding affinity of these three proteins (Wuyts et al. 1999).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to human GRO-gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human GRO-gamma.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- P19876
- Entrez Gene
- CXCL3
- GO Terms
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0008009 chemokine activity
- GO:0030593 neutrophil chemotaxis
- Regulatory
- For research purposes only
Applications of GRO Gamma antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.5 | 2.0ug/ml | |
Functional Assays | 1.0 | 2.0ug/ml | |
Immunohistology - Paraffin 1 | 0.5 | 0.7ug/ml | |
Western Blotting | 0.1 | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for GRO Gamma antibody
-
Kogan-sakin, I. et al. (2009) Prostate stromal cells produce CXCL-1, CXCL-2, CXCL-3 and IL-8 in response to epithelia-secreted IL-1.
Carcinogenesis. 30 (4): 698-705.
Further Reading
-
Haskill, S. et al. (1990) Identification of three related human GRO genes encoding cytokine functions.
Proc Natl Acad Sci U S A. 87 (19): 7732-6. -
Geiser, T. et al. (1993) The interleukin-8-related chemotactic cytokines GRO alpha, GRO beta, and GRO gamma activate human neutrophil and basophil leukocytes.
J Biol Chem. 268 (21): 15419-24.
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