GRO Gamma antibody

Rabbit anti Human GRO Gamma

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
GRO Gamma

Product Code Applications Pack Size List Price Your Price Qty
AHP774
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SDS Safety Datasheet SDS
E FN P * WB 0.1 mg loader
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loader
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Rabbit anti Human GRO gamma antibody recognizes human GRO-gamma, also known as CXCL3, Growth-regulated protein gamma, GRO-gamma(1-73), Macrophage inflammatory protein 2-beta or MIP2-beta. GRO gamma is a ~7.9 kDa CXC chemokine and one of three closely related, but distinct GRO gene products, which act as chemoattractants and activators of neutrophils and basophils.

Like GRO-alpha (CXCL1) and GRO-beta (CXCL2), GRO-gamma contains the ELR-motif (N-Terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors though there are differences in the potency and receptor binding affinity of these three proteins (Wuyts et al. 1999).

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to human GRO-gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human GRO-gamma.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 1.0 2.0ug/ml
Immunohistology - Paraffin 1 0.5 0.7ug/ml
Western Blotting 0.1 0.2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for GRO Gamma antibody

  1. Kogan-sakin, I. et al. (2009) Prostate stromal cells produce CXCL-1, CXCL-2, CXCL-3 and IL-8 in response to epithelia-secreted IL-1.
    Carcinogenesis. 30 (4): 698-705.

Further Reading

  1. Haskill, S. et al. (1990) Identification of three related human GRO genes encoding cytokine functions.
    Proc Natl Acad Sci U S A. 87 (19): 7732-6.
  2. Geiser, T. et al. (1993) The interleukin-8-related chemotactic cytokines GRO alpha, GRO beta, and GRO gamma activate human neutrophil and basophil leukocytes.
    J Biol Chem. 268 (21): 15419-24.

Synonyms
CXCL3
RRID
AB_2230080
UniProt
P19876
Entrez Gene
CXCL3
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
GO:0030593 neutrophil chemotaxis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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