Rabbit anti GRK2 antibody
recognizes beta-adrenergic receptor kinase 1, also known as ADRBK1, BARK or BARK1. It belongs to the family of serine/threonine protein kinases called GPCR kinases (GRKs) that phosphorylate agonist occupied receptors (Pitcher et al. 1998
). PKC-mediated phosphorylation of GRK2 increases activity toward its substrates (Krasel et al. 2001
). GRK2 mediates ERK activation downstream of the EGFR by acting as a Rho-activated scaffold protein (Robinson and Pitcher 2013
). As GRK2 can modulate several hallmarks of cancer by participation in many signaling pathways, it was suggested that it acts as an oncomodifier (Nogues et al. 2017
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to GRK2 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human GRK2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
desensitization of G-protein coupled receptor protein signaling pathway
negative regulation of the force of heart contraction by chemical signal
G-protein coupled receptor kinase activity
signal transducer activity
activation of phospholipase C activity
muscarinic acetylcholine receptor signaling pathway
tachykinin receptor signaling pathway
alpha-2A adrenergic receptor binding
Edg-2 lysophosphatidic acid receptor binding
positive regulation of catecholamine secretion
negative regulation of striated muscle contraction
beta-adrenergic receptor kinase activity
nerve growth factor receptor signaling pathway
cardiac muscle contraction
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of GRK2 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti GRK2 antibody detects a band of approximately 90 kDa in cell and tissue lysates under reducing conditions
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Secondary Antibodies Available
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