GRK2 antibody

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Rabbit anti GRK2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2722
Datasheet
WB
MSDS
50 µl loader

Rabbit anti GRK2 antibody recognizes beta-adrenergic receptor kinase 1, also known as ADRBK1, BARK or BARK1. It belongs to the family of serine/threonine protein kinases called GPCR kinases (GRKs) that phosphorylate agonist occupied receptors (Pitcher et al. 1998). PKC-mediated phosphorylation of GRK2 increases activity toward its substrates (Krasel et al. 2001). GRK2 mediates ERK activation downstream of the EGFR by acting as a Rho-activated scaffold protein (Robinson and Pitcher 2013). As GRK2 can modulate several hallmarks of cancer by participation in many signaling pathways, it was suggested that it acts as an oncomodifier (Nogues et al. 2017).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to GRK2 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human GRK2

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P25098
Entrez Gene
ADRBK1
GO Terms
GO:0002029 desensitization of G-protein coupled receptor protein signaling pathway
GO:0003108 negative regulation of the force of heart contraction by chemical signal
GO:0005829 cytosol
GO:0004703 G-protein coupled receptor kinase activity
GO:0004871 signal transducer activity
GO:0005524 ATP binding
GO:0005625 soluble fraction
GO:0007202 activation of phospholipase C activity
GO:0007213 muscarinic acetylcholine receptor signaling pathway
GO:0007217 tachykinin receptor signaling pathway
GO:0018105 peptidyl-serine phosphorylation
GO:0031694 alpha-2A adrenergic receptor binding
GO:0031755 Edg-2 lysophosphatidic acid receptor binding
GO:0033605 positive regulation of catecholamine secretion
GO:0045988 negative regulation of striated muscle contraction
GO:0047696 beta-adrenergic receptor kinase activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0060048 cardiac muscle contraction
Regulatory
For research purposes only

Applications of GRK2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti GRK2 antibody detects a band of approximately 90 kDa in cell and tissue lysates under reducing conditions

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml