GPI antibody
Goat anti GPI
GPI antibody recognizes glucose-6-phosphate isomerase, also known as GPI, autocrine motility factor, AMF Neuroleukin, NLK phosphoglucose isomerase, PGI Phosphohexose isomerase, PHI Sperm antigen 36 or SA-36. The antibody has been extensively validated for western blotting using whole cell lysates.
GPI Antibody with Control Lysate
GPI antibody recognizes glucose-6-phosphate isomerase, also known as GPI, autocrine motility factor, AMF Neuroleukin, NLK phosphoglucose isomerase, PGI Phosphohexose isomerase, PHI Sperm antigen 36 or SA-36. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00064 | WB | 100 µl | ||||
| VPA00064KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human GPI antibody recognizes glucose-6-phosphate isomerase. GPI is a ubiquitously expressed glycolytic enzyme that catalyzes the conversion of fructose-6-phosphate to glucose-6-phosphate. GPI (or neuroleukin) is also recognized for its neurotrophic effects on spinal and sensory neurons.
Goat anti human GPI antibody detects GPI as a band of 63 kDa in a range of human cell line lysates.
Goat anti human GPI antibody detects GPI as a band of 63 kDa in a range of human cell line lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Goat anti GPI detects a band of approximately 63 kDa in HEK293 cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide with sequence C-RERMFNGEKINYT from the internal region (near N terminus) of the protein sequence.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P06744 Related reagents
- Entrez Gene
- GPI Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0001525 angiogenesis
- GO:0004347 glucose-6-phosphate isomerase activity
- GO:0005829 cytosol
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0005634 nucleus
- GO:0006094 gluconeogenesis
- GO:0006096 glycolysis
- GO:0006959 humoral immune response
- GO:0007599 hemostasis
- GO:0008083 growth factor activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of GPI antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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