GPI antibody

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Goat anti GPI

GPI antibody recognizes glucose-6-phosphate isomerase, also known as GPI, autocrine motility factor, AMF Neuroleukin, NLK phosphoglucose isomerase, PGI Phosphohexose isomerase, PHI Sperm antigen 36 or SA-36. The antibody has been extensively validated for western blotting using whole cell lysates.

GPI Antibody with Control Lysate

GPI antibody recognizes glucose-6-phosphate isomerase, also known as GPI, autocrine motility factor, AMF Neuroleukin, NLK phosphoglucose isomerase, PGI Phosphohexose isomerase, PHI Sperm antigen 36 or SA-36. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00064 WB 100 µl
VPA00064KT WB 2 Westerns
With Lysate 400µg
Goat anti Human GPI antibody recognizes glucose-6-phosphate isomerase. GPI is a ubiquitously expressed glycolytic enzyme that catalyzes the conversion of fructose-6-phosphate to glucose-6-phosphate. GPI (or neuroleukin) is also recognized for its neurotrophic effects on spinal and sensory neurons.

Goat anti human GPI antibody detects GPI as a band of 63 kDa in a range of human cell line lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence C-RERMFNGEKINYT from the internal region (near N terminus) of the protein sequence.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P06744 Related reagents
Entrez Gene
GPI Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001525 angiogenesis
GO:0004347 glucose-6-phosphate isomerase activity
GO:0005829 cytosol
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
GO:0006959 humoral immune response
GO:0007599 hemostasis
GO:0008083 growth factor activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of GPI antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Goat anti GPI detects a band of approximately 63 kDa in HEK293 cell lysates.
Western Blotting
Goat anti GPI detects a band of approximately 63 kDa in HEK293 cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB