Glutathione Reductase antibody

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Rabbit anti Glutathione Reductase

Glutathione Reductase Antibody with Control Lysate

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00735 WB 100 µl
VPA00735KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human glutathione reductase antibody recognizes glutathione reductase, mitochondrial, also known as GR, GRase or epididymis luminal protein 75.

The GSR gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in GSR result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found (provided by RefSeq, Aug 2010).

Rabbit anti Human glutathione reductase antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on Protein A
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography on Protein A
Buffer Solution
HEPES buffered saline
Buffer Solution
HEPES buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
<50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
E. coli-derived recombinant human glutathione reductase

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P00390 Related reagents
Entrez Gene
GSR Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0005739 mitochondrion
GO:0004362 glutathione-disulfide reductase activity
GO:0005829 cytosol
GO:0055114 oxidation-reduction process
GO:0045454 cell redox homeostasis
GO:0015949 nucleobase, nucleoside and nucleotide interconversion
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Glutathione Reductase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Rabbit anti glutathione reductase detects a band of approximately 56 kDa in HeLa cell lysates
Western Blotting
Rabbit anti glutathione reductase detects a band of approximately 56 kDa in HeLa cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB