Glutathione Peroxidase 1 antibody
Rabbit anti Glutathione Peroxidase 1
Rabbit anti-human glutathione peroxidase 1 antibody recognizes glutathione peroxidase 1, also known as GPx-1 or GSHPx-1. This antibody has been extensively validated for western blotting using whole cell lysates.
Glutathione Peroxidase 1 Antibody with Control Lysate
Rabbit anti-human glutathione peroxidase 1 antibody recognizes glutathione peroxidase 1, also known as GPx-1 or GSHPx-1. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00488 | WB | 100 µl | ||||
| VPA00488KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human glutathione peroxidase 1 antibody recognizes glutathione peroxidase 1, also known as GPx-1, GSHPx-1 or cellular glutathione peroxidase.
GPX1 gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for GPX1 (provided by RefSeq, Jul 2008).
Rabbit anti Human glutathione peroxidase 1 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
GPX1 gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for GPX1 (provided by RefSeq, Jul 2008).
Rabbit anti Human glutathione peroxidase 1 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti glutathione peroxidase 1 detects a band of approximately 22 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid.
- Product Form
- Purified IgG - liquid.
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography.
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Immunogen
- KLH conjugated synthetic peptide corresponding to amino acid 164-193 of human glutathione peroxidase 1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Guarantee
- As supplied, 12 months from date of despatch.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P07203 Related reagents
- Entrez Gene
- GPX1 Related reagents
- GO Terms
- GO:0001836 release of cytochrome c from mitochondria
- GO:0005739 mitochondrion
- GO:0004602 glutathione peroxidase activity
- GO:0005829 cytosol
- GO:0004866 endopeptidase inhibitor activity
- GO:0045454 cell redox homeostasis
- GO:0006749 glutathione metabolic process
- GO:0006144 purine base metabolic process
- GO:0006195 purine nucleotide catabolic process
- GO:0006916 anti-apoptosis
- GO:0061136 regulation of proteasomal protein catabolic process
- GO:0009650 UV protection
- GO:0010269 response to selenium ion
- GO:0017124 SH3 domain binding
- GO:0033599 regulation of mammary gland epithelial cell proliferation
- GO:0040029 regulation of gene expression, epigenetic
- GO:0042744 hydrogen peroxide catabolic process
- GO:0043154 negative regulation of caspase activity
- GO:0060047 heart contraction
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only.
Applications of Glutathione Peroxidase 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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