Glutathione Peroxidase 1 antibody

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Rabbit anti Glutathione Peroxidase 1

Rabbit anti-human glutathione peroxidase 1 antibody recognizes glutathione peroxidase 1, also known as GPx-1 or GSHPx-1. This antibody has been extensively validated for western blotting using whole cell lysates.

Glutathione Peroxidase 1 Antibody with Control Lysate

Rabbit anti-human glutathione peroxidase 1 antibody recognizes glutathione peroxidase 1, also known as GPx-1 or GSHPx-1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00488 WB 100 µl
VPA00488KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human glutathione peroxidase 1 antibody recognizes glutathione peroxidase 1, also known as GPx-1, GSHPx-1 or cellular glutathione peroxidase.

GPX1 gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for GPX1 (provided by RefSeq, Jul 2008).

Rabbit anti Human glutathione peroxidase 1 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 164-193 of human glutathione peroxidase 1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P07203 Related reagents
Entrez Gene
GPX1 Related reagents
GO Terms
GO:0001836 release of cytochrome c from mitochondria
GO:0005739 mitochondrion
GO:0004602 glutathione peroxidase activity
GO:0005829 cytosol
GO:0004866 endopeptidase inhibitor activity
GO:0045454 cell redox homeostasis
GO:0006749 glutathione metabolic process
GO:0006144 purine base metabolic process
GO:0006195 purine nucleotide catabolic process
GO:0006916 anti-apoptosis
GO:0061136 regulation of proteasomal protein catabolic process
GO:0009650 UV protection
GO:0010269 response to selenium ion
GO:0017124 SH3 domain binding
GO:0033599 regulation of mammary gland epithelial cell proliferation
GO:0040029 regulation of gene expression, epigenetic
GO:0042744 hydrogen peroxide catabolic process
GO:0043154 negative regulation of caspase activity
GO:0060047 heart contraction
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Glutathione Peroxidase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti glutathione peroxidase 1 detects a band of approximately 22 kDa in Jurkat cell lysates
Western Blotting
Anti glutathione peroxidase 1 detects a band of approximately 22 kDa in Jurkat cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB