Glutamate Dehydrogenase 1 antibody
Rabbit anti Human glutamate dehydrogenase 1 antibody recognizes glutamate dehydrogenase 1, mitochondrial, also known as GDH 1, glutamate dehydrogenase (NAD(P)+), glutamate dehydrogenase 1, mitochondrial.
The GLUD1 gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in GLUD1 are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from GLUD1 via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of GLUD1 are present in humans (provided by RefSeq, Sep 2009).
Rabbit anti Human glutamate dehydrogenase antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The GLUD1 gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in GLUD1 are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from GLUD1 via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of GLUD1 are present in humans (provided by RefSeq, Sep 2009).
Rabbit anti Human glutamate dehydrogenase antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Immunogen
- Synthetic peptide encompassing part of the N terminal region of human glutamate dehydrogenase 1
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P00367 Related reagents
- Entrez Gene
- GLUD1 Related reagents
- GO Terms
- GO:0004352 glutamate dehydrogenase activity
- GO:0004353 glutamate dehydrogenase [NAD(P)+] activity
- GO:0005524 ATP binding
- GO:0005525 GTP binding
- GO:0005759 mitochondrial matrix
- GO:0055114 oxidation-reduction process
- GO:0006537 glutamate biosynthetic process
- GO:0006538 glutamate catabolic process
- GO:0042802 identical protein binding
- GO:0032024 positive regulation of insulin secretion
- GO:0043531 ADP binding
- GO:0070403 NAD+ binding
- GO:0070728 leucine binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Glutamate Dehydrogenase 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Rabbit anti glutamate dehydrogenase 1 detects a band of approximately 52 kDa in HepG2 cell lysates
- Western Blotting
- Rabbit anti glutamate dehydrogenase 1 detects a band of approximately 52 kDa in HepG2 cell lysates
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
