Glutamate Dehydrogenase 1 antibody

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Anti Glutamate Dehydrogenase 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Rabbit anti Human glutamate dehydrogenase 1 antibody (**VPA00667**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to glutamate dehydrogenase 1 (molecular weight 52 kDa).

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Rabbit anti Glutamate Dehydrogenase 1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Glutamate Dehydrogenase 1

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VPA00667 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human glutamate dehydrogenase 1 antibody recognizes glutamate dehydrogenase 1, mitochondrial, also known as GDH 1, glutamate dehydrogenase (NAD(P)+), glutamate dehydrogenase 1, mitochondrial.

The GLUD1 gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in GLUD1 are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from GLUD1 via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of GLUD1 are present in humans (provided by RefSeq, Sep 2009).

Rabbit anti Human glutamate dehydrogenase antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Rabbit anti glutamate dehydrogenase 1 detects a band of approximately 52 kDa in HepG2 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide
2% Sucrose
Immunogen: Synthetic peptide encompassing part of the N terminal region of human glutamate dehydrogenase 1
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P00367
Entrez Gene: GLUD1
GO Terms: GO:0004352 glutamate dehydrogenase activity; GO:0004353 glutamate dehydrogenase [NAD(P)+] activity; GO:0005524 ATP binding; GO:0005525 GTP binding; GO:0005759 mitochondrial matrix; GO:0055114 oxidation-reduction process; GO:0006537 glutamate biosynthetic process; GO:0006538 glutamate catabolic process; GO:0042802 identical protein binding; View More GO Terms; GO:0032024 positive regulation of insulin secretion; GO:0043531 ADP binding; GO:0070403 NAD+ binding; GO:0070728 leucine binding