Glutamate Dehydrogenase 1 antibody

100% Secure

Rabbit anti Glutamate Dehydrogenase 1

Product Type
PrecisionAb Polyclonal
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
100 µl loader

Rabbit anti Human glutamate dehydrogenase 1 antibody recognizes glutamate dehydrogenase 1, mitochondrial, also known as GDH 1, glutamate dehydrogenase (NAD(P)+), glutamate dehydrogenase 1, mitochondrial.

The GLUD1 gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in GLUD1 are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from GLUD1 via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of GLUD1 are present in humans (provided by RefSeq, Sep 2009).

Rabbit anti Human glutamate dehydrogenase antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Western Blotting
Rabbit anti glutamate dehydrogenase 1 detects a band of approximately 52 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Synthetic peptide encompassing part of the N terminal region of human glutamate dehydrogenase 1

Storage Information

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0004352 glutamate dehydrogenase activity
GO:0004353 glutamate dehydrogenase [NAD(P)+] activity
GO:0005524 ATP binding
GO:0005525 GTP binding
GO:0005759 mitochondrial matrix
GO:0055114 oxidation-reduction process
GO:0006537 glutamate biosynthetic process
GO:0006538 glutamate catabolic process
GO:0042802 identical protein binding
GO:0032024 positive regulation of insulin secretion
GO:0043531 ADP binding
GO:0070403 NAD+ binding
GO:0070728 leucine binding
PrecisionAb is a trademark of Bio-Rad Laboratories
For research purposes only

Applications of Glutamate Dehydrogenase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra