Glutamate Decarboxylase 1 antibody
Rabbit anti Human glutamate decarboxylase 1 antibody recognizes glutamate decarboxylase 1, also known as 67 kDa glutamic acid decarboxylase, GAD-67 and GAD1.
GAD1 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of GAD1 results in two products, the predominant 67 kDa form and a less-frequent 25 kDa form (provided by RefSeq, Jul 2008).
Rabbit anti Human glutamate decarboxylase 1 antibody detects a band of 67 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
GAD1 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of GAD1 results in two products, the predominant 67 kDa form and a less-frequent 25 kDa form (provided by RefSeq, Jul 2008).
Rabbit anti Human glutamate decarboxylase 1 antibody detects a band of 67 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti glutamate decarboxylase 1 antibody recognizes a band of approximately 67 kDa in NIH/3T3 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Immunogen
- Synthetic peptide corresponding to a portion of the N terminal region of human glutamate decarboxylase 1
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q99259
- Entrez Gene
- GAD1
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0042136 neurotransmitter biosynthetic process
- GO:0004351 glutamate decarboxylase activity
- GO:0006540 glutamate decarboxylation to succinate
- GO:0007269 neurotransmitter secretion
- GO:0018352 protein-pyridoxal-5-phosphate linkage
- GO:0030170 pyridoxal phosphate binding
- GO:0061202 clathrin sculpted gamma-aminobutyric acid transport vesicle membrane
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Glutamate Decarboxylase 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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