Glutamate Decarboxylase 1 antibody

Rabbit anti Glutamate Decarboxylase 1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Glutamate Decarboxylase 1

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VPA00827
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Rabbit anti Human glutamate decarboxylase 1 antibody recognizes glutamate decarboxylase 1, also known as 67 kDa glutamic acid decarboxylase, GAD-67 and GAD1.

GAD1 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of GAD1 results in two products, the predominant 67 kDa form and a less-frequent 25 kDa form (provided by RefSeq, Jul 2008).

Rabbit anti Human glutamate decarboxylase 1 antibody detects a band of 67 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti glutamate decarboxylase 1 antibody recognizes a band of approximately 67 kDa in NIH/3T3 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Immunogen
Synthetic peptide corresponding to a portion of the N terminal region of human glutamate decarboxylase 1
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q99259
Entrez Gene
GAD1
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0042136 neurotransmitter biosynthetic process
GO:0004351 glutamate decarboxylase activity
GO:0006540 glutamate decarboxylation to succinate
GO:0007269 neurotransmitter secretion
GO:0018352 protein-pyridoxal-5-phosphate linkage
GO:0030170 pyridoxal phosphate binding
GO:0061202 clathrin sculpted gamma-aminobutyric acid transport vesicle membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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